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Reagents, kits, and instruments for use during the extraction of proteins from bacteria, cultured mammalian cells and tissues, insect cells, yeast cultures, flowering plants, etc. Products include lysis buffers, master mixes, and solubilizing solutions.
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
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1% SDS Lysis Buffer is great for fully solubilizing proteins in preparation for Western blotting! It is suitable for both organs and tissue culture cells. Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.
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The R-PLEX Hu Cathepsin B Total Assay is a multiplex immunoassay kit from Meso Scale Diagnostics that allows for the quantitative measurement of total human Cathepsin B in cell and tissue lysates It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and low sample consumption The assay requires 50 L of sample and can be read on Meso Scale Diagnostics SECTOR Imagers The kit includes a 96-well plate coated with capture antibody detection antibody Read Buffer Calibrator Diluent wash buffer and lyophilized calibrator
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