Bioluminescence Assays and Reagents
Imanis Life Sciences Cell Line, Human, B Lymphoblast, Fluc, EmGFP
This human B-lymphoblast cell line, derived from Raji cells (ATCC® CCL-86™), expresses enhanced firefly luciferase (Fluc) and emerald green fluorescent protein (EmGFP). It is generated using a self-inactivating (SIN) lentiviral vector for enhanced biosafety.
- Suitable for in vitro and in vivo studies.
- Fluc enables noninvasive bioluminescent imaging of implanted cells.
- EmGFP facilitates postmortem analysis by fluorescence microscopy or flow cytometry.
- Mycoplasma free.
- Grows in RPMI-1640 Medium with 10% FBS and 1% Penicillin/Streptomycin.
- Maintain density between 5 x 105 and 2 x 106 cells/mL.
- Characterized for morphology, reporter expression, and in vivo growth.
- Biosafety level 2 reagents.
- Store below -130°C in vapor phase above liquid nitrogen.
Sigma Aldrich Fine Chemicals Biosciences firefly Luciferase Assay f
firefly luciferase is widely used as a reporter for studying gene regulation and function and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. firefly luciferase is a 62 000 Dalton protein which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. The firefly Luciferase Assay is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working SOLUTIONon to samples. The luminescence signal decays over the course of about 10 minutes of reaction
HORIZON DISCOVERY BIOSCIENCES LIMITED SMVC INDUC NONTARG CTRL 2X25UL
NC3725785 SMVC INDUC NONTARG CTRL 2X25UL
