General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress CXCR3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0054E2CP2L)
The cAMP Hunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR3, a naturally Gi/Go coupled GPCR. Upon activation of CXCR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CXCR2 (IL8RB) CHO-K1 GPCR Assay, 2-Plate Kit. (95-0074E2CP2M)
The cAMP Hunter CXCR2 (IL8RB) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of CXCR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR2, a naturally Gi/Go coupled GPCR. Upon activation of CXCR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human BMP-6 ASY
The R-PLEX Human BMP-6 Assay Kit from Meso Scale Discovery is an electrochemiluminescent immunoassay for the quantitative measurement of human Bone Morphogenetic Protein 6 (BMP-6) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SECTOR Imager 6000 for high sensitivity detection The kit includes a 96-well plate coated with capture antibody detection antibody calibrator and sample diluent The assay has a range from 1 5 pg/mL to 1000 pg/mL and takes approximately 3 5 hours to complete This product can be used for biomarker research related to bone formation fracture healing and developmental disorders
Meso Scale Discovery RPLEX HUM PRAS40 total ASY
The R-PLEX Human PRAS40 Total Assay Kit from Meso Scale Discovery is used to quantitatively measure levels of total PRAS40 protein in human cell and tissue samples The assay utilizes an electrochemiluminescent detection method and provides high sensitivity broad dynamic range and requires low sample input The kit includes a 96-well plate coated with capture antibody detection antibody read buffer sample diluent and vials of calibrator This assay allows for the simple and rapid analysis of PRAS40 protein levels which can provide insight into mTOR pathway biology and related disease states
Meso Scale Discovery R-PLEX Human Trk-A total ASY
The R-PLEX Human TrkA Total Assay Kit from Meso Scale Discovery measures total TrkA in human cell and tissue lysates This electrochemiluminescent immunoassay uses MSD s SULFO-TAG labels and multi-array plate technology for high sensitivity and minimal matrix interference The kit includes a 96-well plate with capture antibody detection antibody with ruthenium label calibrators and necessary reagents Results are available in 3 5 hours using an MSD instrument This assay accurately quantifies total TrkA levels
Meso Scale Discovery R-PLEX Human Tenascin C ASY
The R-PLEX Human Tenascin-C Assay from Meso Scale Discovery is an electrochemiluminescent immunoassay designed for the quantitative measurement of Tenascin-C in human serum plasma and cell culture supernatants This assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and rapid detection of Tenascin-C an extracellular matrix glycoprotein implicated in tissue injury and cancer progression With a detection range of 1 56-400 ng/mL and minimal required sample volume this assay allows high-throughput and accurate quantification of Tenascin-C levels from small sample sizes
Eurofins Discoverx PathHunter eXpress CCR7 HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0195E1CP0M)
The PathHunter CCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of CCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR7 and EA-tagged β-Arrestin-2. Activation of CCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Methylated DNA Quantification Kit (Colorimetric)
Methylated DNA Quantification Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress NTSR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0671E3CP5M)
The PathHunter NTSR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NTSR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged NTSR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting NTSR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress C5L2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0645E2CP0L)
The PathHunter C5L2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C5a) based activation of C5L2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C5L2 and EA-tagged β-Arrestin-2. Activation of C5L2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR27 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0334E2ACP1M)
The PathHunter GPR27 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR27 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR27 and EA-tagged β-Arrestin-2. Activation of GPR27 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GHSR1b CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0354E2ACP1L)
The PathHunter GHSR1b eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ghrelin) based activation of GHSR1b GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GHSR1b and EA-tagged β-Arrestin-2. Activation of GHSR1b stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human VEGF-a ASY
The Meso Scale Discovery U-PLEX Human VEGF-A Assay Kit is a multiplex immunoassay for measuring VEGF-A in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and broad dynamic range with low sample volume The assay offers high specificity and low cross-reactivity It includes a plate with capture antibodies detection antibodies with electrochemiluminescent labels calibrator diluent wash buffer read buffer and a 10-point VEGF-A standard curve The kit is customizable with a 3 5-hour testing time suitable for biomarker research drug discovery and clinical research
