General Cell Based Assays
Eurofins Discoverx PathHunter Human SSTR3 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human SSTR3 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure SSTR3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human NTSR1 Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human NTSR1 Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure NTSR1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human Calcitonin (CALCR (No RAMP)) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human Calcitonin (CALCR (No RAMP)) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CALCR (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress PROKR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0282E2CP2M)
The PathHunter PROKR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PROKR2 and EA-tagged β-Arrestin-2. Activation of PROKR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Medchemexpress LLC cGAS Human His-SUM 10ug
Recombinant human cyclic GMP-AMP synthase (cGAS) fragment G161-F522, expressed in E. coli and supplied lyophilized with an N-terminal 6xHis-SUMO tag. The protein is purified to >90% and provided with storage and reconstitution guidance to preserve activity for biochemical and structural applications.
- Recombinant human cGAS fragment G161-F522 with N-terminal His-SUMO tag.
- Expressed in E. coli and purified to greater than 90% purity.
- Lyophilized formulation for improved stability and convenient storage.
- Buffer contains trehalose to protect protein during lyophilization.
- Suitable for biochemical assays, enzymatic activity, and structural studies.
- Includes storage and reconstitution guidance to maintain activity.
Abcam Cathepsin B Assay Kit (Magic Red)
Cathepsin B Assay Kit (Magic Red)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress mCCR7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0528PE2MCP0L)
The PathHunter mCCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of mCCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR7 and EA-tagged β-Arrestin-2. Activation of mCCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
AURORA BIOLABS LLC PKMYT1 Binding Assay, 384 rxn
A fluorescence-labelled tracer, that can bind to PKMYT1, and the N-terminal GST-tagged full-length human PKMYT1 are used in this assay kit. A Terbium-labeled anti-GST antibody binding to the GST-PKMYT1 serves as a fluorescence donor (HTRF donor). If the fluorescence-labeled tracer binds to the PKMYT1, the binding brings Terbium on the anti-GST antibody close to the fluorophore on the tracer (HTRF acceptor). Activation of the Terbium results in fluorescence resonance energy transfer (FRET). Thus, the binding status can be quantitively measured by calculating the ratio of the emission fluorescence intensity of the acceptor (665 nm) and donor (620nm). The competitive binding of a non-fluorescence-labeled compound will reduce the FRET signal.
American Research Products Inc Human EXtracellular superoXide
Human EXtracellular superoXide dismutase [Cu-Zn] (SOD3) ELISA kit from CUSABIO Detection Range 7 8 pg/mL-500 pg/mL Sensitivity 1 95 pg/mL Sample serum plasma cell culture supernates tissue homogenates saliva urine Method Sandwich
Eurofins Discoverx HitHunter cAMP Assay for Biologics, 200/800 dp (96-well/384-well). (90-0075LM2)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 2 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
CPC Scientific H-Thr-Ser-Gly-Pro-Leu-Gly-Asn-Leu-Ala-Glu-Glu-Leu-Asn-Gly-Tyr-Ser-Arg-Lys-Lys-Gly-Gly-Phe-Ser-Phe-Arg-Phe-NH2 (trifluoroacetate salt) 1MG
An RF-amide peptide ligand for SP9155, an orphan G-protein-coupled receptor with high homology to orexin, neuropeptide FF and CCK receptors. It has been shown to potently activate SP9155 with an EC50 of 7 nM.ONE-LETTER SEQUENCE: TSGPLGNLAEELNGYSRKKGGFSFRF-NH2MOLECULAR FORMULA: C127H195N37O37MOLECULAR WEIGHT:2832.17STORAGE CONDITIONS: -20 5C, CAS REGISTRY NUMBER: [600171-68-4], RESEARCH AREA: PainREFERENCES: N.Chartrel et al., Proc. Natl. Acad. Sci. USA, 100, 15247 (2003); Y.Jiang et al., J. Biol. Chem., 278, 27652 (2003)
Eurofins Discoverx cAMP Hunter eXpress MTNR1A CHO-K1 GPCR Assay, 10-Plate Kit. (95-0086E2CP2L)
The cAMP Hunter MTNR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melatonin) based activation of MTNR1A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MTNR1A, a naturally Gi/Go coupled GPCR. Upon activation of MTNR1A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human ADGRB3 (BAI3) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human ADGRB3 (BAI3) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure BAI3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Discovery Life Sciences Gentest CynoMonkey Hep
Gentest Cynomolgus Monkey Hepatocytes Cynomolgus Cryopreserved Suspension Female
Montana Molecular Borealis Arrestin AT1R Assay - 10mL
Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Angiotensin II Type I receptor (AT1R) assay. Add the unmodified AT1R and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, AT1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). Borealis kits for several other receptors are also available, and larger-sized kits are available on request. Other Montana Molecular tools including the DAG and Calcium sensors can be used alongside this assay.