General Cell Based Assays
Meso Scale Discovery R-PLEX Human N-Cadherin ASY
The R-Plex Human N-Cadherin Assay is a multiplex immunoassay kit for measuring N-Cadherin in human serum plasma and cell culture supernatants It uses a quantitative sandwich enzyme immunoassay and color-coded microspheres to measure multiple biomarkers in one well The kit includes microplates detection antibodies standards controls diluents wash solutions substrates and stop solutions for 96 tests With a range of 1 56 - 400 ng/mL and sensitivity of 0 64 ng/mL it ensures sensitive reproducible and accurate quantification of N-Cadherin levels
Eurofins Discoverx PathHunter eXpress VIPR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0739E3CP5M)
The PathHunter VIPR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of VIPR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged VIPR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting VIPR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Darbepoetin Alfa Bioassay Kit, 2pl. (93-0965Y3-00019)
The PathHunter Darbepoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoetin or darbepoetin alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The cell line included overexpresses EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress TAAR5 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0718E2CP1L)
The PathHunter TAAR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of TAAR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TAAR5 and EA-tagged β-Arrestin-2. Activation of TAAR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Discovery Life Sciences Gentest CD 1 Mouse Hepatocytes
Gentest CD 1 Mouse Hepatocytes Cryopreserved Male Suspension
Eurofins Discoverx PathHunter OSMRb/IL31R Bioassay Kit, 2-Plate. (93-1002Y3-00083)
The PathHunter OSMRb/IL31R Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-31) induced dimerization of OSMRb/IL31R receptors. This product uses EFC technology. The cell line included overexpresses OSMRb target protein fused to PK alongside IL31RA target protein fused to EA. Upon ligand-induced dimerization of OSMRb/IL31R, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical 2 3cyclIc NADP sodIum sa 7.3mg
A substrate for CNP; has been used in coupled enzyme assays to quantify CNP activity; increases calcium overload-induced calcium release and prevents calcium-induced swelling in rat brain mitochondria at 5 µM
Discovery Life Sciences Gentest CD 1 Mouse Hep
Gentest CD 1 Mouse Hepatocytes Plateable Inducible Grade Female
Eurofins Discoverx PathHunter eXpress TYK2 Activity Assay, 2-Plate Kit. (93-0875E3CP16M)
The PathHunter eXpress TYK2 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the TYK kinase domain. The cell line is engineered to express the catalytic domain from TYK2 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the TYK2 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 21 Reagent, 500. (93-0563R21B)
AssayComplete Cell Plating 21 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 50 (1,000 dp, 96-well). (93-1043)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 10 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043.
Eurofins Discoverx PathHunter eXpress TGFBR1/ACVR2 Dimerization Assay, 10pl Kit. (93-1012E3CP5L)
The PathHunter TGFBR1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF-11) induced dimerization of TGFBR1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human DPPIV ASY
The Meso Scale Discovery U-PLEX Human DPP4 Assay is a multiplex immunoassay panel that measures Dipeptidyl Peptidase 4 (DPP4) in human serum plasma and cell culture supernatants This assay utilizes MSD s electrochemiluminescent detection technology and is designed for use on MSD s QuickPlex SQ 120 instrument The assay has high sensitivity and wide dynamic range It requires a low sample volume of 50 L The total assay time is approximately 3 5 hours This product provides a fast simple and robust way to measure DPP4 levels across multiple samples
Eurofins Discoverx AssayComplete Cell Plating 3 Reagent, 500. (93-0563R3B)
AssayComplete Cell Plating 3 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress CCR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0651E3CP5L)
The PathHunter CCR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.