General Cell Based Assays
Cayman Chemical PhagocytosIs asy KItIgG F 1ea
Cayman’s Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-FITC Complex for up to 750 samples.
Cell Applications Inc Rat Cardiac Fibroblast Total Kit, fetal
Rat Cardiac Fibroblast Total Kit, fetal:(R306-05f) Cryopreserved (RCF), 1 vial, fetal(R116-500) Rat Fibroblast Growth Media, 500 ml(090K) Subculture Reagent Kit
Meso Scale Discovery R-PLEX Human N-Cadherin ASY
The R-Plex Human N-Cadherin Assay is a multiplex immunoassay kit for measuring N-Cadherin in human serum plasma and cell culture supernatants It uses a quantitative sandwich enzyme immunoassay and color-coded microspheres to measure multiple biomarkers in one well The kit includes microplates detection antibodies standards controls diluents wash solutions substrates and stop solutions for 96 tests With a range of 1 56 - 400 ng/mL and sensitivity of 0 64 ng/mL it ensures sensitive reproducible and accurate quantification of N-Cadherin levels
Eurofins Discoverx PathHunter Human Activin B (ACVR1C/ACVR2) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human Activin B (ACVR1C/ACVR2) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of ACVR1C/ACVR2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Discovery Life Sciences Gentest CD 1 Mouse Hep
Gentest CD 1 Mouse Hepatocytes Plateable Inducible Grade Female
Eurofins Discoverx PathHunter Human VEGF (KDR/KDR) Dimerization Cell-Based Assay Kit (HEK 293)
The PathHunter Human VEGF (KDR/KDR) Stable Cell Line Dimerization Assay (HEK 293) measures ligand-induced dimerization of KDR/KDR. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress ADRB2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0182E2CP0L)
The PathHunter ADRB2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRB2 and EA-tagged β-Arrestin-2. Activation of ADRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Heme Assay Kit sufficient
Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm and is a key prosthetic group for various essential proteins such as hemoglobin cytochromes catalases and peroxidases. free heme which can be released from hemoglobin following hemolysis is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.
Eurofins Discoverx PathHunter eXpress PTGER3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0640E2CP0L)
The PathHunter PTGER3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER3 and EA-tagged β-Arrestin-2. Activation of PTGER3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BioVendor, LLC Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Large Economy Kit Size: 400 assays), Multispecies colorimetric assay, Biocolor Item # S6000
Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Large Economy Kit Size: 400 assays). The Sircol; INSOLUBLE Collagen Assay is a dye-binding method for analysis of insoluble collagen fibers. The assay be used to assess the rate of production of newly laid down collagen fibers during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue. It replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis! The Sircol Insoluble Assay uses mild acid and temperature treatment for 2 to 3 hrs. The solubility conversion to denatured collagen and subsequent measurement can be completed within one working day. The ratio of soluble and insoluble collagens can be measured using the same Sircol Dye Reagent. Multispecies colorimetric assay. Detection Limit: 10.0 g. Assay-time: 4 hours. Biocolor Item # S6000
Abcam SIRT2 Activity Assay Kit (Fluorometric)
SIRT2 Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery R-PLEX Rat IL-2 ASY
The R-PLEX Rat IL-2 Assay Kit from Meso Scale Discovery measures rat interleukin 2 (IL-2) in various biological fluids using MSD s electrochemiluminescent detection technology It offers high sensitivity a wide dynamic range and low sample volume requirements The kit includes a 96-well plate with capture antibodies detection antibodies calibrators and necessary reagents Results are available in 3 5 hours using an MSD instrument This assay quantitatively measures rat IL-2 crucial for the immune system
Eurofins Discoverx AssayComplete Cell Plating 21 Reagent, 500. (93-0563R21B)
AssayComplete Cell Plating 21 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
BioVendor, LLC QuickZyme Sensitive Tissue Hydroxyproline Kit 2 X 96-Assays (for collagen analysis)
QuickZyme Sensitive Tissue Hydroxyproline Kit, 2 X 96-Assays. Measure hydroxyproline in small tissue samples with low collagen levels! The ONLY hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate, enabling measurement in small samples with low hydroxyproline levels. No drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Dynamic range: 6-300 uM. Sensitivity: 0.3 ug/ml. Species independent. Measures total hydroxyproline. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all collagen types present without discriminating between types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Collagen hydrolysis tubes & protocol not included (if these items are needed, please order QuickZyme Sensitive Tissue Collagen Assay).
Cayman Chemical Methyltrnsferase asy AdoHc 1ea
An ago-PAM of GLP-1R induces calcium mobilization in 9-3-H cells expressing human GLP-1R (EC50 1.3 UM) enhances GLP-1- exenatide- or liraglutide-induced calcium mobilization in the same cells (EC50s 1.8 8.4 and 30 UM respectively) potentiates exenatide-induced insulin secretion in primary mouse pancreatic islets at 30 UM reverses haloperidol-induced catalepsy in a rat model of Parkinson’s disease motor symptoms at 30 mg/kg