General Cell Based Assays

A broad array of cell-based assays designed for use with a variety of live cell experiments and procedures. Ideal for molecular and cell biology research applications.

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2,146 – 2,160 of 6,698 results

2,146

Amsbio LLC Heparanase Assay Kit with Positive Control Enzyme

Heparanase Assay Kit with Enzyme as Positive Control

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2,147

Biolegend Alexa FluorR 594 anti-beta-act

Alexa Fluor 594 anti-β-actin [2F1-1]; Isotype: Mouse IgG2b, κ; Reactivity: Human, Mouse, Rat; Apps: ICC, IHC-P; Size: 100 μg

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2,148

AnaSpec SensoLyte Red Glucocerebrosidase (GBA) Assay Kit Fluorimetric

The SensoLyte Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. The GBA substrate provided in the kit releases the red fluorescent dye resorufin upon Glucocerebrosidase cleavage with absorption/emission maxima at 570 nm/610 nm. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds.

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2,149

Cayman Chemical Glycolysis Cell-Based Assay Kit, 1 ea

Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase (LDH) catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.

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2,150

Revvity Health Sciences Inc HTRF mAb Anti-6His XL665-Conjugate, 5,000 Assay Points

HTRF mAb Anti-6His XL665-Conjugate, 5,000 Assay Points

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2,151

Abcam L-Lactate Assay Kit (Colorimetric/Fluorometric)

L-Lactate Assay Kit (Colorimetric/Fluorometric)

The product is subject to the following: Abcam Restricted Use Statement

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2,152

AdipoGen Asprosin human ELISA Kit

Colorimetric Sandwich ELISA Assay. Detects human asprosin. Assay Type Sandwich. Detection Type Colorimetric. Sample Type Cell Culture Supernatant, Plasma, Serum. Range 0.3125 to 20ng/ml. Sensitivity 230pg/ml. Asprosin is a new fasting-induced protein hormone that targets the liver to increase plasma glucose levels. Asprosin is the C-terminal cleavage product of the protein Fibrillin-1. Asprosin is secreted from white adipose tissue and increases hepatic glucose production by using cAMP as a second messenger, leading to activation of protein Kinase A. Reduction of asprosin levels protects against metabolic syndrome-associated hyperinsulinism. Asprosin may act as a circulating hunger signal. Indeed, peripherally injected recombinant asprosin can cross the blood-brain barrier and intracerebroventricular i.c.v. injection of recombinant asprosin stimulated appetite in wild-type mice, indicating a central mechanism of action.

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2,153

Eurofins Discoverx PathHunter eXpress CHRM1 Activated GPCR Internalization Assay, 10pl Kit. (93-0655E3CP5L)

The PathHunter CHRM1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.