General Cell Based Assays
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter eXpress DRD2S (Short Isoform) CHO-K1 GPCR Assay, 10pl Kit. (95-0084E2CP2L)
The cAMP Hunter DRD2S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD2S, a naturally Gi/Go coupled GPCR. Upon activation of DRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences MMP-1 Assay Buffer (20ml)
Formulation: Liquid. In 50mM HEPES, pH 7.5, 10mM CaCl2 and 0.05% Brij-35. Long Term Storage: Ambient.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 2-Plate. (95-0118Y2-00057)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Enzo Life Sciences FLUOR DE LYS HDAC fluorometric activity assay kit (96 wells)
First-to-market by the leader in Epigenetics research tools. Synonyms: Histone deacetylase fluorescent assay kit. Applications: Fluorescent detection, HTS, Activity assay.
Invivogen InvivoGen PD-1/PD-L1 Bio-IC™ Assay, Bioluminescent Cell-Based System, Mycoplasma-Free, 2 Cryovials
The InvivoGen PD-1/PD-L1 Bio-IC™ assay is a bioluminescent, cell-based system designed for screening antagonists of the PD-1/PD-L1 immune checkpoint axis. It provides a reliable mechanism-of-action approach, eliminates the need for primary cell culture, and is mycoplasma-free, ensuring consistent results. This assay mimics the immune synapse, allowing for accurate evaluation of antagonist potency through quantifiable bioluminescent signals.
Dojindo Molecular Technologies Inc GSSG/GSH Quantification Kit (200 TESTS) is high reproducibility for detecting GSH, GSSG and total glutathione individually. Easy and simple procedure, takes 1-2 hours.
Glutathione is a tripeptide present in the body, involved in antioxidation, drug metabolism, as well as enzyme substrates of glutathione peroxidase, glutathione S-transferase, and thiol transferase, etc. Glutathione is usually present as reduced form (GSH), but GSH is converted into its oxidized form (GSSG) by stimulation such as oxidative stress. Therefore, the ratio of GSH and GSSG has been noted as an index of oxidative stress. The GSSG/GSH Quantification kit contains the Masking Reagent of GSH. The GSH can be deactivated in the sample by simply adding the Masking Reagent. Therefore, the GSSG is detected by measuring the absorption (λmax = 412 nm) of DTNB (5,5 Edithiobis (2-nitrobenzoic acid) ) using the enzymatic recycling system. Also, GSH can be determined by the quantity by subtracting GSSG from the total amount of glutathione. The kit is limited to quantifying GSH/GSSG concentration from 0.5 μmol/l to 50 μmol/l and GSSG concentration from 0.5 μmol/l to 25 μmol/l.
BioVendor, LLC QuickZyme Sensitive Tissue Collagen Assay Kit 5 X 96-Assays
QuickZyme Sensitive Tissue Collagen Assay Kit, 5 X 96-Assays. Measure hydroxyproline in small tissue samples with low collagen levels! The ONLY hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate, enabling measurement in small samples with low hydroxyproline levels, such as liver. No drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Dynamic range: 6-300 ug/ml. Sensitivity: 2.5 ug/ml. Sample vol. 50-500 ul. Species independent. Measures total hydroxyproline. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all collagen types present without discriminating between types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Collagen hydrolysis tubes & protocol ARE included. Ease-of-use: Equivalent to ELISA.
Enzo Life Sciences SOD activity kit (5x96wells)
ELS offers a large collection of superoxide dismutase products for oxidative stress research. This is a versatile assay for the determination of SOD activity. Differentiate between the activity of three SOD isoenzymes: MnSOD, FeSOD, and Cu/ZnSOD. Obtain higher throughput sample analysis with a convenient colorimetric 96 well plate format and fast results in just 10 minutes. Alternative Name: Superoxide dismutase. Applications: Colorimetric detection, Activity assay. Contents: Microtiter plate, Standard, SOD buffer concentrate, Xanthine oxidase, Xanthine solution, Triton x-100, WST-1 reagent