General Cell Based Assays
Abcam Caspase-3 Assay Kit (Colorimetric)
Caspase-3 Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress TACR2 Activated GPCR Internalization Assay, 2pl Kit. (93-0677E3CP0M)
The PathHunter TACR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of TACR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged TACR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting TACR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM5 Activated GPCR Internalization Assay, 10pl Kit. (93-0657E3CP5L)
The PathHunter CHRM5 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TACR2 Activated GPCR Internalization Assay, 10pl Kit. (93-0677E3CP0L)
The PathHunter TACR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of TACR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged TACR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting TACR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human PYY active ASY
The R-PLEX Hu PYY Active Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures active PYY levels in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker solutions calibrator and controls The active assay specifically measures the biologically active form of PYY and allows sensitive reproducible quantitation of PYY levels
Meso Scale Discovery R-PLEX Mouse M-CSF ASY
The R-PLEX Mouse M-CSF Assay from Meso Scale Diagnostics is a multiplex immunoassay kit for measuring mouse macrophage colony stimulating factor (M-CSF) in various biological fluids This ECL-based assay uses MULTI-ARRAY technology and includes a pre-coated 96-well plate calibrators controls diluent detection antibody read buffer and seals It has a sensitivity of 0 3 pg/mL and an upper limit of 10 000 pg/mL The assay offers high sensitivity low sample volume and quick results
Meso Scale Discovery RPLEX NHP Alphaamylase 2A ASY
The R-Plex NHP Alpha-Amylase 2A Assay Kit from Meso Scale Discovery is a 96-well electrochemiluminescent immunoassay for the quantitative measurement of alpha-amylase 2A in human serum plasma saliva and cell culture supernatants The assay uses MSD GOLD SULFO-TAG labels and has a typical sensitivity of 0 04 ng/mL with an assay range of 0 1-400 ng/mL The kit includes a 96-well MULTI-ARRAY Human Alpha-Amylase 2A Plate SULFO-TAG Anti-Alpha-Amylase 2A Antibody Sample Diluent Read Buffer T (4X) and validations packet This assay allows for high-throughput sensitive and accurate quantification of alpha-amylase 2A levels
Meso Scale Discovery U-PLEX Mouse SDF-1 ASY
The Meso Scale Discovery U-PLEX Mouse SDF-1alpha Assay is a multiplex immunoassay kit that enables simultaneous quantification of mouse SDF-1alpha in serum plasma and cell culture supernatants It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision with a quick 2 5 hour turnaround time per 96-well plate This assay requires a Meso Scale Discovery SECTOR Imager and provides components for one 96-well plate including a pre-coated plate detection antibody read buffer calibrator and controls
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 50 (1,000 dp, 96-well). (93-1043)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 10 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043.
Meso Scale Discovery R-PLEX Human N-Cadherin ASY
The R-Plex Human N-Cadherin Assay is a multiplex immunoassay kit for measuring N-Cadherin in human serum plasma and cell culture supernatants It uses a quantitative sandwich enzyme immunoassay and color-coded microspheres to measure multiple biomarkers in one well The kit includes microplates detection antibodies standards controls diluents wash solutions substrates and stop solutions for 96 tests With a range of 1 56 - 400 ng/mL and sensitivity of 0 64 ng/mL it ensures sensitive reproducible and accurate quantification of N-Cadherin levels
Dojindo Molecular Technologies Inc SOD Assay Kit-WST (500 TESTS) is a colorimetric microplate and low background noise measurement which measures 100% inhibition by SOD.
Superoxide dismutase (SOD) is a crucial antioxidative enzyme, which catalyzes the dismutation of the superoxide anion (O2-) into hydrogen peroxide and molecular oxygen. In order to determine SOD activity, several direct and indirect methods have been developed. Among these methods, the indirect method using nitroblue tetrazolium (NBT) is commonly used due to the convenience and ease to use. However, using the NBT method yields poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. SOD Assay Kit-WST allows for very convenient SOD assays by utilizing Dojindo's highly water-soluble tetrazolium salt, WST-1, producing a water-soluble formazan dye upon reduction with the superoxide anion. The rate of the reduction of WST-1 with O2- is linearly related to the xanthine oxidase (XO) activity depending on SOD. Therefore, the IC50 (50% inhibition activity of SOD or SOD-like materials) can be determined by the colorimetric method.
Eurofins Discoverx PathHunter SSTR2 Bioassay Kit, 2-Plate. (93-0181Y2-00067)
The PathHunter SSTR2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. somatostatin 28) based activation of SSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR2 and EA-tagged β-Arrestin-2. Activation of SSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress CCR5 Activated GPCR Internalization Assay, 10pl Kit. (93-0653E3CP0L)
The PathHunter CCR5 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.