General Cell Based Assays
Sigma Aldrich Fine Chemicals Biosciences In Vitro Toxicology Assay
Tox7 is a member of Sigmas CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays as well as conventional cytotoxicity resulting from interaction of a test material with the cell.
Sigma Aldrich Fine Chemicals Biosciences Ammonia Assay Kit sufficie
Ammonia (NH3) or the ammonium ion (NH4 ) is found in the atmosphere rainwater soil seawater and volcanic areas. It is widely used as fertilizer being an important source of nitrogen for living systems. It also plays a role in animal physiology. Ammonia is produced by amino acid metabolism and is converted to urea in the human liver through the urea cycle. Ammonia acts as a precursor for various other nitrogen compounds. It is a colorless gas with pungent odor and can be easily liquefied. It is also used as a feed supplement in livestock industry.
Sigma Aldrich Fine Chemicals Biosciences Superoxide Dismutase SOD A
Superoxide dismutase (SOD) is part of a family of metal ion-containing enzymes (SODs) that are found in most organisms from bacteria to mammals. SODs catalyze the dismutation of superoxide (generated by aerobic respiration) to molecular oxygen and peroxide. Superoxide dismutase form the front line of defence against reactive oxygen species (ROS)-mediated injury and harbor anti-inflammatory activities. The Superoxide Dismutase (SOD) Activity Assay Kit provides a simple and sensitive procedure for measuring SOD enzymatic activity in various sample types. SOD activity is determined by measuring the decrease in superoxide anions (generated by the enzyme xanthine oxidase [XO]). Since superoxide anions interact with the provided WST dye yielding color at 450 nm the decrease in the color signal is proportional to SOD inhibition activity. The kit allows the determination of SOD activity in percent inhibition or in units of activity using the provided SOD Enzyme as a standard. The provided
Sigma Aldrich Fine Chemicals Biosciences Cholesterol Quantification
Cholesterol is a lipid that constitutes 20-25% of the structural components of cell membranes. Cholesterol presence assure membrane structural fluidity. Cholesterol also plays a role as an intermediate in several hormones and bile acid biosynthesis and vitamin D. Cholesterol also regulates the functions of the transporters and signaling proteins present on the plasma membrane. The major sites of cholesterol synthesis are the small intestine and liver. Cholesterol circulates in the blood as both the free acid and as cholesterol esters. Increased cholesterol levels are associated with the development of atherosclerosis and cardiovascular diseases. Thus serum cholesterol regulation has an important therapeutic role.The Cholesterol Quantification Assay Kit provides a simple and quick procedure for detecting and measuring very low concentrations of Cholesterol. This kit can measure free cholesterol cholesteryl esters or total cholesterol (which constitutes both free cholesterol and chole
Eurofins Discoverx cAMP Hunter eXpress AGTRL1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0147E2CP2L)
The cAMP Hunter AGTRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type AGTRL1, a naturally Gi/Go coupled GPCR. Upon activation of AGTRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL10RA/10RB Dimerization Bioassay Kit, 2pl. (93-0985Y3-00135)
The PathHunter IL10RA/IL10RB Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-10) induced dimerization of IL10RA/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IL10RA target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IL10RA/IL10RB, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress cINSRa Functional Assay, 10-Plate Kit. (93-1016E3CP16L)
The PathHunter eXpress cINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged cINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY4 Activated GPCR Internalization Assay, 2pl Kit. (93-0741E3CP0M)
The PathHunter P2RY4 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY4 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY4. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY4-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1A CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0696E2CP0M)
The PathHunter HTR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1A and EA-tagged β-Arrestin-2. Activation of HTR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXTR Activated GPCR Internalization Assay, 10pl Kit. (93-0715E3CP0L)
The PathHunter OXTR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OXTR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged OXTR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting OXTR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.