General Cell Based Assays
Eurofins Discoverx PathHunter eXpress JAK3 Activity Assay, 2-Plate Kit. (93-0796E3CP16M)
The PathHunter eXpress U2OS JAK3 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the JAK3 kinase domain. The cell line is engineered to express the catalytic domain from JAK3 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the PRLR-PK. Activation of the PRLR-PK induces receptor phosphorylation by the JAK3 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human VEGF-a ASY
The Meso Scale Discovery U-PLEX Human VEGF-A Assay Kit is a multiplex immunoassay that measures VEGF-A levels in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay has high specificity and low cross-reactivity It includes a plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrator and buffer The kit allows quantification of VEGF-A levels with a lower limit of detection of 0 06 pg/mL
Eurofins Discoverx PathHunter eXpress cyno GPR120 CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-1029E2CP0L)
The PathHunter cyno GPR120 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. free fatty acids) based activation of cyno GPR120 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno GPR120 and EA-tagged β-Arrestin-2. Activation of cyno GPR120 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL6RA/IL6ST Dimerization Assay, 10pl Kit. (93-1045E3CP5L)
The PathHunter IL6RA/IL6ST Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-6) induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6RA target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human TACR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human TACR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure TACR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TRHR Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human TRHR Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure TRHR (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical MItochndral CMPLX ASSAY 10mL
A G protein-biased agonist of GPR84 inhibits forskolin-induced cAMP production in CHO cells expressing human GPR84 (EC50 0.00598 nM) does not induce B-arrestin recruitment at concentrations up to 80 UM
Cayman Chemical LIPID Drop asSAy Wash Sol 30mL
A BET BD2 inhibitor (IC50s 31.62 15.85 15.85 and 39.81 nM for BRD2 BRD3 BRD4 and BRDTBD2 respectively) selective for BD2 in BRD2 BRD3 BRD4 and BRDT over BD1 in the same BRDs (IC50s >10000 nM for all)
Revvity Health Sciences Inc HTRF Mouse TNF-α Detection Kit, 10,000 Assay Points
HTRF Mouse TNF-α Detection Kit, 10,000 Assay Points
Eurofins Discoverx PathHunter eXpress CXCR2 Activated GPCR Internalization Assay, 10pl Kit. (93-0934E3CP5L)
The PathHunter CXCR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0850
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0850
Meso Scale Discovery R-PLEX Human ADAM17 ASY
The R-PLEX Human ADAM17 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures active ADAM17 in human serum plasma and cell culture supernatants The assay utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity a broad dynamic range and requires a low sample volume The kit includes a 96-well plate coated with capture antibody a detection antibody and calibrator The assay can detect ADAM17 levels between 1 5-1000 pg/mL and each kit provides sufficient reagents for 1 x 96-well plate This product enables the accurate quantitation of ADAM17 which plays a key role in regulating EGFR signaling
Eurofins Discoverx cAMP Hunter™ Human MC5R Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human MC5R Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure MC5R (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.