General Cell Based Assays
Eurofins Discoverx HitHunter cAMP Assay for Biologics, 200/800 dp (96-well/384-well). (90-0075LM2)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 2 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
ELABSCIENCE Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method) (E-BC-K261-M) 96 tests
Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of trinder’s reagent, hydrogen peroxide is catalyzed by peroxidase to produce quinones which is proportional to the content of TG.
Eurofins Discoverx AssayComplete Thawing Reagent T3, 100. (92-4103TR)
AssayComplete Thawing Reagent T3 is an optimized thawing reagent for revival and recovery of Eurofins DiscoverX cell lines. This reagent ensures optimal post-thaw viability, recovery and assay performance upon thawing. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended thawing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter eXpress DRD2S (Short Isoform) CHO-K1 GPCR Assay, 2pl Kit. (95-0084E2CP2M)
The cAMP Hunter DRD2S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD2S, a naturally Gi/Go coupled GPCR. Upon activation of DRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Glycerol Assay Kit sufficient for 200 colorimetric or fluorometric tests | 1KT
Glycerol Assay Kit sufficient for 200 colorimetric or fluorometric tests | 1KT
Eurofins Discoverx PathHunter eXpress SSTR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0181E2CP0M)
The PathHunter SSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR2 and EA-tagged β-Arrestin-2. Activation of SSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BDKRB2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0189E2CP0M)
The PathHunter BDKRB2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Brookfield Engineer Labs VIAL TESTING ASSAY KIT 10PK
DescriptionGlass Sample bottles with metal cap and septa. Compatible with Vapor Pro instruments CT-3100(-L) and CT-4200XL. Includes 10 glass sample bottles.
Eurofins Discoverx PathHunter eXpress SSTR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0181E2CP0L)
The PathHunter SSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR2 and EA-tagged β-Arrestin-2. Activation of SSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TARGETMOL CHEMICALS INC P38 MAPK INHIBITOR 5MG
Also available in 1 mg 10 mg 25 mg 50 mg 100 mg 500 mg and bulk. Please contact Fisher for quotes. p38 MAPK inhibitor is a potent inhibitor of p38 MAP kinase (IC50 = 35 nM). It inhibits senescence induced by the oncogene RAS. purity: 99%