General Cell Based Assays
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-ERK (Thr202/Tyr204) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-ERK (Thr202/Tyr204) Detection Kit, 10,000 Assay Points
VAZYME INTERNATIONAL LLC ADP-Lite Kinase Assay
ADP-Lite Kinase Assay is a universal kinase activity assay kit designed for measuring the activity of any ADP-generating enzyme (e.g., kinases and ATPases). To accurately determine target kinase activity, the assay quantifies the ADP produced during a kinase reaction, which is positively correlated with the kinase activity. This simple, rapid, and homogeneous assay is based on a highly sensitive in vitro bioluminescence technology that requires no excitation light or radioactive materials. It is compatible with a wide range of kinase substrates, including lipids, peptides, proteins, and carbohydrates.The kit contains two core components: ADP-Lite Reagent (for ATP depletion) and Kinase Detection Reagent (for ADP detection). Kinase Detection Reagent is prepared by mixing Kinase Detection Buffer and Kinase Detection Substrate (Lyophilized).
VAZYME INTERNATIONAL LLC Add Read cAMP Detection Kit 1 Kit
cAMP Detection Kit is a competitive immunoassay intended to measure cyclic AMP (cAMP) accumulation in cells. The kit contains an antibody that specifically recognizes cAMP, labeled with a fluorescent donor Eu (Anti-cAMP-Eu), and cAMP is labeled with a fluorescent acceptor A2 (cAMP-A2). In the solution, Anti-cAMP-Eu binds with cAMP-A2, which can lead to Fluorescence Resonance Energy Transfer (FRET). When excited with light at 320 nm, the fluorescent donor Eu emits light at 620 nm. This 620 nm light, in turn, excites the fluorescent acceptor A2, causing it to emit light at 665 nm. After cell lysis, the cAMP produced within the cells is released into the lysate sample, which competes for binding with Anti-cAMP-Eu, disrupting the FRET phenomenon.The FRET signal value (the ratio of light intensity at 665 nm to 620 nm) is inversely proportional to the concentration of cAMP in the standard or sample.
GINKGO BIOWORKS INC (3P) 1 mL CFPS kit. 100 reactions of 10 uL. Contains lysate, reagent mix, and control plasmid.
Ginkgo’s cell free protein synthesis (CFPS) kit is an E. coli transcription/translation system optimized to produce proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification.1 mL kit enables user to run 100 reactions of 10 µL, 20 reactions of 50 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C.
Apexbio Technology LLC Red Fluorometric Lipid Droplet Assay Kit 100T
Red Fluorometric Lipid Droplet Assay Kit is a fluorescent detection kit designed for the visualization and quantification of intracellular lipid droplets It is intended to probe and monitor lipid metabolism by specifically labeling intracellular neutral lipid accumulations This kit exerts its function primarily through the use of Nile Red a cell-permeable lipophilic dye that selectively accumulates within neutral lipid environments resulting in red fluorescence upon excitation (552 nm emission 636 nm) No key metric (IC50/EC50) or specific cell line information is available Based on these properties Red Fluorometric Lipid Droplet Assay Kit enables research applications in cellular lipid metabolism autophagy cell differentiation and senescence
BioVendor, LLC Sircol™ Collagen Isolation & Concentration Reagent (3 x 20 ml)
Sircol™ Collagen Isolation & Concentration Reagent (3 x 20 ml). Contains polyethylene glycol in a TRIS-HCl buffer, pH 7.6. Sircol Collagen Assay component. Biocolor Item # SICR
Eurofins Discoverx cAMP Hunter eXpress ADRB1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0113E2CP2L)
The cAMP Hunter ADRB1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB1, a naturally Gs coupled GPCR. Upon activation of ADRB1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc Rat inositol 1 4 5 trispho
Rat inositol 1 4 5 -trisphosphate (IP3) ELISA kit from CUSABIO Detection Range 5 pg/mL-1000 pg/mL Sensitivity 2 5 pg/mL Sample serum plasma tissue homogenates cell culture supernates cell lysates Method Competitive
Genscript Corporation ToxinSensor Single Tests Kit
GenScript ToxinSensor Single Tests Kit with Standard is designed to be a qualitative In Vitro end-point endotoxin test for human and animal parenteral drugs biological products and medical devices Tachypleus Amebocyte Lysate (TAL) as supplied is to be reconstituted with sample or control directly After incubation for 1 hour and in the presence of endotoxin gelation occurs in the absence of endotoxin gelation does not occur GenScript supply a series of ToxinSensorTM Single Tests Kits with Standard with different sensitivity (0 03 EU/ml 0 06 EU/ml 0 125 EU/ml and 0 25 EU/ml)