General Cell Based Assays
Bioassay Systems EnzyChrom™ NADP/NADPH Assay Kit. For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts.
For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts. Key Features: Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Colorimetric Determination at 565 nm. Samples: Cells, Tissue. Species: human, mouse, rat and more. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 µM.
Bioassay Systems EnzyChrom™ Glucose Oxidase Assay Kit. For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.
For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: Cell/tissue lysate, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 (OD), 0.002U/L (FL).
Proteintech Group Inc Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method)
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
Proteintech Group Inc Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method)
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
Cayman Chemical Phosphatidylcholine Colorimet
Cayman’s PC Assay provides a specific, sensitive, and convenient method for quantifying PC in plasma or serum. In this assay, PC-Specific PLD hydrolyzes PC to choline and phosphatidic acid. The newly formed choline is then used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue dye with an optimal absorption at 595 nm.
TOPOGEN INC DNA Gyrase Drug Screening Kit
This drug screening Kit is based on gyrase supercoiling action on a relaxed plasmid DNA substrate provided (pHOT1, which is a derivative of pBR322) and roughly 2.7 KB. One unit of gyrase will supercoil 200-500 ng in 1 hr at 37oC. The enzyme introduces negative supercoils into a relaxed pHOT1 plasmid substrate via a sign inversion model. An energy co-factor (ATP) is required for the complete supercoiling reaction. Non-EB gels are ideal for detecting gyrase activity as attested by the excellent resolution between supercoiled and relaxed DNA forms. It is designed to identify IFP and CIC (poisons and inhibitors, respectively). A typical IFP (e.g., a fluoroquinolone) will induce double strand DNA breaks while a prototypical CIC will simply block the enyzme from acting on relaxed DNA (novobiocin, some intercalators for example). Thus, an IFP (ciprofloxacin) will result in formation of a linear DNA product while a CIC will block supercoiling activity.
