General Cell Based Assays
Eurofins Discoverx PathHunter eXpress AVPR1A U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0587E3CP0L)
The PathHunter AVPR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR1A and EA-tagged β-Arrestin-2. Activation of AVPR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IFNGR1/IFNGR2 Dimerization Assay, 2pl Kit. (93-1098E3CP5M)
The PathHunter IFNGR1/IFNGR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IFN-g) induced dimerization of IFNGR1/IFNGR2 receptors. This product uses EFC technology. The cell line included overexpresses IFNGR1 target protein fused to PK alongside IFNGR2 target protein fused to EA. Upon ligand-induced dimerization of IFNGR1/IFNGR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 Activated GPCR Internalization Assay, 2pl Kit. (93-0710E3CP0M)
The PathHunter AGTRL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of AGTRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 7 Reagent, 500. (93-0563R7B)
AssayComplete Cell Plating 7 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter Liraglutide Bioassay Kit, 10-Plate. (95-0062Y2-00100)
The cAMP Hunter Liraglutide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Liraglutide based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter GLP2R Bioassay Kit, 10-Plate. (93-0572Y2-00134)
The PathHunter GLP2R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. GLP2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress CHRM2 Activated GPCR Internalization Assay, 2pl Kit. (93-0656E3CP5M)
The PathHunter CHRM2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GLP1R CHO-K1 β-Arrestin-1 GPCR Assay, 2-Plate Kit. (93-0914E2CP2M)
The PathHunter GLP1R (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-1. Activation of GLP1R stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM1 Activated GPCR Internalization Assay, 2pl Kit. (93-0655E3CP5M)
The PathHunter CHRM1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter PL/PK Detection kit, 4,000 dp (384-well). (93-0812)
The PathHunter ProLabel/PK Detection kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cell lines or with custom cell lines overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 10 plates (96/384-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0812.
Eurofins Discoverx PathHunter eXpress CCR2 Total GPCR Internalization Assay, 10pl Kit. (93-0679E3CP5L)
The PathHunter CCR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CCR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GLP2R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0112E2CP2L)
The cAMP Hunter GLP2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP2R, a naturally Gs coupled GPCR. Upon activation of GLP2R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GLP1R Total GPCR Internalization Assay, 10pl Kit. (93-1075E3CP5L)
The PathHunter GLP1R Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GLP1R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged GLP1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GLP1R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter TrkB-P75 Bioassay Kit, 2-Plate. (93-0530Y3-00075)
The PathHunter TrkB-P75 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. BDNF) induced dimerization of TrkB-P75 receptors. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Upon ligand-induced dimerization of TrkB-P75, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.