General Cell Based Assays
IPHASE PHARMA SERVICES LLC 5α-Reductase(SRD5A1) Inhibition Kit,200 test (Micropore)
Mainly used in 5α-Reductase(SRD5A2) Inhibition study. It's recommended to purchase the complete reagent kit.
Eurofins Discoverx PathHunter eXpress GPR27 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0334E2ACP1L)
The PathHunter GPR27 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR27 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR27 and EA-tagged β-Arrestin-2. Activation of GPR27 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 10 (200 dp, 96-well). (93-1043E)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 2 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043e.
Eurofins Discoverx PathHunter IL17RA/IL17RC Dimerization Bioassay Kit, 2pl. (93-0999Y3-00053)
The PathHunter IL17RA/IL17RC Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-17) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 50 (1,000 dp, 96-well). (93-1043)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 10 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043.
Meso Scale Discovery R-PLEX Human U-PAR ASY
The R-PLEX Human U-PLEX Assay is a multiplex immunoassay panel that measures 38 biomarkers related to inflammation immune response and tissue remodeling It utilizes electrochemiluminescence detection technology on the Meso Scale Discovery platform The assay requires 50 L of sample and can be used with serum plasma and cell culture supernatants It has high sensitivity broad dynamic range and requires low sample volume The panel includes cytokines chemokines growth factors soluble receptors and matrix metalloproteinases It is designed for applications in immunology oncology neurobiology cardiovascular and metabolic research
Eurofins Discoverx PathHunter eXpress C5AR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0557E2CP0L)
The PathHunter C5AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TIM3/TIM3 Dimerization Assay, 10-Plate Kit. (93-1082E3CP5L)
The PathHunter TIM3/TIM3 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand induced dimerization of TIM3/TIM3 receptors. This product uses EFC technology. The cell line included overexpresses TIM3 target protein fused to PK alongside TIM3 target protein fused to EA. Upon ligand-induced dimerization of TIM3/TIM3, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0181E2CP0L)
The PathHunter SSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR2 and EA-tagged β-Arrestin-2. Activation of SSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx HitHunter cGMP Assay, 2,400 dp (384-well). (90-0039M)
The HitHunter cGMP Assay is a detection kit offering a robust, sensitive and easy-to-use assay to measure intracellular cGMP levels. This product uses EFC technology, and contains ED-tagged cGMP, anti-cGMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cGMP, the anti-cGMP antibody will be sequestered by binding to this untagged cGMP, allowing for EFC between the tagged cGMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit may be used with Eurofins DiscoverX cell lines as well as cell lines from other sources. This detection kit contains reagents for 6 plates (384 well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter IGF1R Bioassay Kit, 10-Plate. (93-0505Y1-00070)
The PathHunter IGF1R Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. IGF1, IGF2, agonist antibody) based activation of IGF1R receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged IGF1R target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx AssayComplete Cell Plating 9 Reagent, 500. (93-0563R9B)
AssayComplete Cell Plating 9 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter Exenatide Bioassay Kit, 10-Plate. (95-0062Y2-00102)
The cAMP Hunter Exenatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exenatide based activation of GLP1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery U-PLEX Human IL-1 ASY
The Meso Scale Discovery U-PLEX Human IL-1alpha Assay Kit is a multiplex immunoassay that measures IL-1alpha in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The assay can quantify IL-1alpha concentrations between 0 06 pg/mL to 10 000 pg/mL It includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and an MSD read buffer The total assay time is 3 5 hours