General Cell Based Assays
Cayman Chemical Aconitase Assay Kit, 96 Well
Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. Cayman’s Aconitase Assay provides a simple, reproducible, and sensitive tool for assaying aconitase from tissue homogenates or cell lysates.
Eurofins Discoverx PathHunter eXpress PTHR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0315E2CP0L)
The PathHunter PTHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PTH) based activation of PTHR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR1 and EA-tagged β-Arrestin-2. Activation of PTHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR109A CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0301E2CP2L)
The PathHunter GPR109A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109A and EA-tagged β-Arrestin-2. Activation of GPR109A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR135 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0431E2ACP1L)
The PathHunter GPR135 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR135 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR135 and EA-tagged β-Arrestin-2. Activation of GPR135 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR65 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0428E2ACP1M)
The PathHunter GPR65 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR65 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR65 and EA-tagged β-Arrestin-2. Activation of GPR65 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR176 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0382E2ACP1M)
The PathHunter GPR176 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR176 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR176 and EA-tagged β-Arrestin-2. Activation of GPR176 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cambridge Isotope Laboratories GLYCAN-F6-13C-GAL4 (GALACTOSE-13C6, 99%), 500 PMOL
GLYCAN-F6-13C-GAL4 (GALACTOSE-13C6, 99%), 500 PMOL
TRIBIOSCIENCE INC Total Antioxidant Capacity (TAC)- CUPRAC Colorimetric Assay, 200 Tests
TribioScience’s Total Antioxidant Capacity Colorimetric Assay provides a quick, efficient, and sensitive method to measure TAC from cell lysate, plasma serum, urine, tissue homogenates, and food extracts. This assay is based on the reduction of Cu2+ to Cu+ by antioxidants like Trolox. The resulting Cu+ specifically forms a colored complex with a colorimetric probe. The color intensity at 490nm is proportional to TAC in the sample.
Revvity Health Sciences Inc HTRF Human Total Phospholamban Detection Kit, 50,000 Assay Points
HTRF Human Total Phospholamban Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-IRF3 (Ser386) Detection Kit, 500 Assay Points
HTRF Human Phospho-IRF3 (Ser386) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total Phospholamban Detection Kit, 10,000 Assay Points
HTRF Human Total Phospholamban Detection Kit, 10,000 Assay Points
Eurofins Discoverx PathHunter Mouse CRHR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse CRHR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CRHR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cell Biolabs Inc NAD+/NADH Assay Kit (Colorimetric), 100 assays
Cell Biolabs’ NAD+/NADH Assay Kit (Colorimetric) is a simple assay that measures NAD+ and NADH present in biological samples such as cell lysates or tissue extracts in a 96-well microtiter plate format. The kit is specific for NAD+, NADH, and their ratio. The kit will not detect NADP+ or NADPH. The total NAD+/NADH concentrations of unknown samples are determined by comparison with a known NAD+ standard. Determination of both NAD+ and NADH requires two separate samples for quantification. NAD+ and NADH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 4 nM NAD+. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NAD+ standards and unknown samples.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-TBK1 (Ser172) Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Phospho-TBK1 (Ser172) Detection Kit, 50,000 Assay Points