General Cell Based Assays
Eurofins Discoverx PathHunter Human GPR52 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human GPR52 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure GPR52 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0557
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0557
Bioassay Systems EnzyLight™ ATP Assay Kit. For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.
For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ATP or a single cell can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µM.
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0739
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0739
Codex Biosolutions Inc MITOCHONDRIAL MEMB IND KIT 10P
Mitochondrial Membrane Potential Indicator Kit 10 plates
Indigo Biosciences INDIGO Biosciences Human c-MET / HGFR Reporter Assay System, 384-well, Bio-luminescence
This all-inclusive reporter assay system is for quantifying functional interactions against c-MET or the coupled RAS-MAPK pathway. It uses human cells engineered to express Human c-MET and a luciferase reporter gene, providing a sensitive measure of drug-induced changes in c-MET activity.
- Utilizes human cells engineered to express c-MET and a luciferase reporter gene.
- Quantifies luciferase activity to measure drug-induced c-MET activity.
- Cryo-preservation enables immediate dispensing of reporter cells.
- Includes reporter cells, media, activator, detection reagents, and a 384-well assay plate.
- Bio-luminescence technology offers low background, high-sensitivity, and broad linear dynamic range.
BPS Bioscience Inc UTX (KDM6A) Homogeneous Assay Kit, 384 reactions
The UTX (KDM6A) Homogenous Assay Kit is designed to measure activity of the UTX (Ubiquitously transcribed tetratricopeptide repeat, X chromosome, also known as KDM6A) for screening and profiling purposes. The kit comes in a convenient format 384-well AlphaLISA® format, with enough purified recombinant UTX (amino acids 1-1401), primary antibody, substrate and buffers for 400 enzyme reactions.
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0733
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0733
Cayman Chemical Glucose-6-Phosphate Fluorometric Assay Kit, 96 Well
Glucose-6-phosphate (G6P) plays a key role in the homeostatic regulation of blood glucose levels by participating in glycolysis, glycogen synthesis, gluconeogenesis, and can be metabolized to NADPH, enabling protection against oxidative damage. Disruption of G6P activity leads to glycogen storage disease type I or von Gierke’s disease, a group of inherited metabolic diseases characterized by severe hypoglycemia, growth retardation, and hepatomegaly, due to accumulation of glycogen and fat in the liver. Cayman’s Glucose-6-Phosphate Fluorometric Assay provides a fluorescence-based method for detecting G6P in tissue homogenates and cell culture samples. In the assay, G6PDH catalyzes the oxidation of G6P to 6-phospho-D-gluconate, along with the concomitant reduction of NADP+ to NADPH. NADPH reacts with the fluorometric detector to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Revvity Health Sciences Inc HTRF Human Phospho-AKT1/2/3 (Thr308) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-AKT1/2/3 (Thr308) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Tau Aggregation Kit, 500 Assay Points
HTRF Tau Aggregation Kit, 500 Assay Points
AURORA BIOLABS LLC Kras Q61H Nucleotide Exchange Assay Kit,100 rxn
KRAS is a small GTPase in the RAS protein family that plays a critical role in regulating cell signaling pathways involved in proliferation, differentiation, and survival. KRAS cycles between an inactive GDP-bound state and an active GTP-bound state, and activation occurs through nucleotide exchange from GDP to GTP. Mutations such as KRAS Q61H are frequently associated with human cancers and contribute to abnormal signaling and uncontrolled cell growth. The KRAS Q61H Nucleotide Exchange Assay is a TR-FRET–based assay designed to quantitatively monitor nucleotide exchange activity of KRAS Q61H. The assay detects changes in fluorescence resonance energy transfer generated during nucleotide exchange, enabling measurement of KRAS activation and evaluation of compounds that modulate KRAS signaling for cancer research and drug discovery applications.
Eurofins Discoverx PathHunter Human Erythropoietin (EpoR/EpoR) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human Erythropoietin (EpoR/EpoR) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of EpoR/EpoR. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress MTNR1A CHO-K1 GPCR Assay, 10-Plate Kit. (95-0086E2CP2L)
The cAMP Hunter MTNR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melatonin) based activation of MTNR1A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MTNR1A, a naturally Gi/Go coupled GPCR. Upon activation of MTNR1A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.