General Cell Based Assays
Meso Scale Discovery R-PLEX Human BMP-5 ASY
The R-PLEX Human BMP-5 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures BMP-5 levels in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator diluent and lyophilized BMP-5 calibrator The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL It can be used to measure BMP-5 levels in biological samples for research applications
Eurofins Discoverx PathHunter eXpress IL2RB/IL2RG/IL2RA Dimerization Assay, 2pl Kit. (93-1003E3CP0M)
The PathHunter IL2RB/IL2RG/IL2RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-2) induced dimerization of IL2RB/IL2RG/IL2RA receptors. This product uses EFC technology. The cell line included overexpresses IL2RB target protein fused to PK alongside IL2RG target protein fused to EA and untagged ILR2A. Upon ligand-induced dimerization of IL2RB/IL2RG/IL2RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Estradiol 17-(β-D-Glucuronide) (sodium salt hydrate), Purity: ≥95%, 50 mg
An estrogen metabolite that acts as a substrate of MRP2 (Km = 75 µM), and through MRP2-mediated transport, functions as a cholestatic agent, decreasing bile flow
Cayman Chemical Lipoxygenase Inhibitor Screening Assay Kit, 96 Well
Cayman’s Lipoxygenase Inhibitor Screening Assay Kit detects and measures the hydroperoxides produced in the lipoxygenation reaction using a purified LO. The detection reaction is equally sensitive to hydroperoxides at various positions within the fatty acid, and will work with fatty acids of any carbon length. It is thus a general detection method for LO, and can be used to screen libraries of compounds for those which inhibit LO enzymes.Provided in this kit is arachidonate 15-LO purified from soybean, which can be used to screen test compounds. Alternatively, if other purified LOs (provided by the user) are to be used for screening, the included enzyme can be used as a control. Both arachidonic and linoleic acids are included as substrates. The preferred substrate for screening will depend on the LO being tested. It is recommended to use the fatty acid substrate best suited for the LO being tested.
American Research Products Inc AMERICAN RESEARCH PRODUCTS INC
5000612574 LPO/LACTOPEROXIDASE ELISA KIT
Eurofins Discoverx PathHunter eXpress NTSR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0671E3CP5L)
The PathHunter NTSR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NTSR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged NTSR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting NTSR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADRA2B CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0641E2CP2M)
The PathHunter ADRA2B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA2B and EA-tagged β-Arrestin-2. Activation of ADRA2B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Mitochondrial Membrane Pot
Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores leading to the release of cytochrome c into the cytosol which in turn triggers other downstream events in the apoptotic cascade.
Miltenyi Biotec, Inc. Memory B Cell Isolation Kit, mouse
Isolation of memory B cells from mouse spleen or lymph nodes.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Eurofins Discoverx PathHunter eXpress mCCR4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0515E2MCP0L)
The PathHunter mCCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL22/MDC) based activation of mCCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR4 and EA-tagged β-Arrestin-2. Activation of mCCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc CytoSelect™ 24-Well Cell Contraction Assay Kit (Floating Matrix Model), 24 assays
Several culture models exist to study fibroblasts' ability of to reorganize and contract collagen matrices in vitro. In the floating matrix contraction model, a freshly polymerized collagen matrix containing cells is released from the culture dish and allowed to float in culture medium, and contraction occurs in the absence of external mechanical load and without appearance of stress fibers in the cells. The CytoSelect™ 24-Well Cell Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators using the floating matrix model. The kit's proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate.