General Cell Based Assays
Biolegend Alexa FluorR 647 anti-AMPKalph
Alexa Fluor 647 anti-AMPKα Phospho (Thr172) [A20017A]; Isotype: Mouse IgG1, κ; Reactivity: Human, Mouse; Apps: IHC-P, ICFC; Size: 100 μg
Cayman Chemical Total nd DIrect BIlIrubIn Co
Cayman’s Total and Direct Bilirubin Colorimetric Assay provides a rapid, reliable, and reproducible colorimetric method for measuring total and direct bilirubin levels in serum. This kit uses a modified Jendrassik-Gróf method, in which total bilirubin concentration is determined by diazo salt in the presence of a catalyst and direct bilirubin is determined in the absence of the catalyst.{55801,55802} The Jendrassik-Gróf method is a widely used clinical method.{55806} However, the results may not be comparable to the results of analytical methods such as mass spectrometry or NMR, and this kit is designed for research use only (not to be used for clinical purposes). The assay has a dynamic range of 0.045-8 mg/dl (0.022-4 μg/well) for both total and direct bilirubin.
Eurofins Discoverx cAMP Hunter eXpress CNR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0183E2CP2L)
The cAMP Hunter CNR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of CNR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CNR2, a naturally Gi/Go coupled GPCR. Upon activation of CNR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD3(1,2) Bromodomain Assay, 10-plate Kit. (96-0038E1CP7L)
The InCELL Hunter eXpress HEK 293 BRD3(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD3(1,2) via detection of BRD3(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRD3(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Montana Molecular TMPRSS2 BacMam - 30mL
TMPRSS2 packaged in BacMam for expression in mammalian cells. BacMam provides even, near 100% transduction efficiency in HEK293 cells as well as expression in many other cell lines, iPSCs, and primary cultures including A549s and HUVECs. Expression is easily titrateable to your experimental needs or to adjust assay sensitivity. This kit includes the TMPRSS2 BacMam and 500mM sodium butyrate in H2O. Use with other Montana Molecular tools including Pseudo-SARS-CoV-2 and BacMam-packaged ACE2, Neuropilin-1, and Cathepsin-L.
Eurofins Discoverx InCELL Hunter eXpress BTK Kinase Binding Assay, 2pl Kit. (96-0056E15CP0M)
The InCELL Hunter eXpress BTK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BTK via detection of BTK levels. This product uses EFC technology. The cell line included overexpresses BTK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BioVendor, LLC FASTIN ELASTIN 110 ASSAYS STNDSZE
TypeColorimetric assayApplicationsCell culture and/or animal studies, Tissue extract, CellSample RequirementsA sample volume of between 50 and 500 μl is required, containing not less than 5 μg and not more than 70 μg elastin.ShippingAt ambient temperature. Upon receipt, store the product at the temperature recommended below.Storage/ExpirationStore the kit at RT or 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box). Calibration Range5 – 70 μgLimit of Detection5 μg
Cayman Chemical MItoLIteRed FX600 500test
A mitochondrial dye for live and fixed cells; ex/em = 575/600 nm
American Research Products Inc Bovine LPO ELISA Kit
This Bovine Lactoperoxidase (LPO) ELISA Kit is a precoated Sandwich ELISA assay designed to detect and quantify the target analyte in milk tears and other biological fluids This product is for Research Use Only
Eurofins Discoverx InCELL Hunter eXpress HCK Kinase Binding Assay, 10pl Kit. (96-0041E15CP0L)
The InCELL Hunter eXpress HCK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of HCK via detection of HCK levels. This product uses EFC technology. The cell line included overexpresses HCK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.