General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress ADCYAP1R1 (PAC1) CHO-K1 GPCR Assay, 2pl Kit. (95-0064E2CP2M)
The cAMP Hunter ADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of ADCYAP1R1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADCYAP1R1, a naturally Gs coupled GPCR. Upon activation of ADCYAP1R1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CCR5 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0068E2CP2M)
The cAMP Hunter CCR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL3) based activation of CCR5 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR5, a naturally Gi/Go coupled GPCR. Upon activation of CCR5, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc NAD+/NADH Assay Kit (Fluorometric), 100 assays
Cell Biolabs’ NAD+/NADH Assay Kit (Fluorometric) is a simple assay that measures NAD+ and NADH present in biological samples such as cell lysates or tissue extracts in a 96-well fluorescence plate reader. The kit is specific for NAD+, NADH, and their ratio. The kit will not detect NADP+ or NADPH. The total NAD+/NADH concentrations of unknown samples are determined by comparison with a known NAD+ standard. Determination of both NAD+ and NADH requires two separate samples for quantification. NAD+ and NADH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 0.8 nM NAD+. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NAD+ standards and unknown samples.
Eurofins Discoverx PathHunter Human GPR88 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR88 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR88 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress mCCR2 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0543E3MCP5M)
The PathHunter mCCR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL2/MCP-1) based activation of mCCR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR2 and EA-tagged β-Arrestin-2. Activation of mCCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MRGPRX4 CHO-K1 β-Arrestin Orphan GPCR Assay, 10pl Kit. (93-0541E2ACP1L)
The PathHunter MRGPRX4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of MRGPRX4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX4 and EA-tagged β-Arrestin-2. Activation of MRGPRX4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
![Biolegend Brilliant Violet 750™ anti-mouse CD185 (CXCR5) [L138D7]; Isotype: Rat IgG2b, κ; Reactivity: Mouse; Apps: FC; Size: 50 ug](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502385182.PNG-250.jpg)
Biolegend Brilliant Violet 750™ anti-mouse CD185 (CXCR5) [L138D7]; Isotype: Rat IgG2b, κ; Reactivity: Mouse; Apps: FC; Size: 50 ug
Brilliant Violet 750™ anti-mouse CD185 (CXCR5) [L138D7]; Isotype: Rat IgG2b, κ; Reactivity: Mouse; Apps: FC; Size: 50 ug
Meso Scale Discovery U-PLEX Human IL-17A/F ASY
The Meso Scale Discovery U-PLEX Human IL-17A/F Assay Kit is a multiplex immunoassay that measures IL-17A and IL-17F in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay takes 3 5 hours to complete and includes a plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels and calibrator and control solutions It allows simultaneous quantification of IL-17A and IL-17F from a single sample for understanding Th17 responses
Eurofins Discoverx PathHunter Human MRGPRD β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human MRGPRD β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure MRGPRD (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human Fractalkine (CX3CR1) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human Fractalkine (CX3CR1) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CX3CR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human DRD5 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human DRD5 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure DRD5 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Human M-CSF ASY 25 PL
The Meso Scale Discovery U-PLEX Human M-CSF Assay is a multiplex immunoassay kit for quantifying macrophage colony-stimulating factor (M-CSF) in human serum plasma and cell culture supernatants It uses MSD s electrochemiluminescent detection technology offering high sensitivity a dynamic range of up to 5 logs and low sample volume needs The 30-plex kit includes a 96-well plate with capture antibodies detection antibodies and necessary calibrators and controls It detects M-CSF with a lower limit of 45 4 pg/mL and an upper limit of 100 000 pg/mL
Eurofins Discoverx PathHunter Human HRH1 (H1) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human HRH1 (H1) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure HRH1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TACR3 Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human TACR3 Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure TACR3 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PTHR2 Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human PTHR2 Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure PTHR2 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.