General Cell Based Assays
Meso Scale Discovery U-PLEX Mouse MIP-1 ASY
The Meso Scale Discovery U-PLEX Mouse MIP-1alpha Assay Kit is a multiplex immunoassay that quantitatively measures mouse MIP-1alpha in serum plasma and cell culture supernatants It utilizes electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The kit includes a plate coated with capture antibodies detection antibodies and calibrators ranging from 3 2 pg/mL to 100 000 pg/mL
Meso Scale Discovery RPLEX MSE Osteoprotegerin ASY
The Meso Scale Discovery R-PLEX Mouse Osteoprotegerin Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of mouse osteoprotegerin (OPG) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The assay has a lower limit of detection of 0 04 ng/mL for mouse OPG and a typical assay time of 3 5 hours This assay enables researchers to accurately quantify circulating OPG levels in mouse models
Meso Scale Discovery U-PLEX Mouse IL-4 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-4 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 4 (IL-4) in various samples It uses MSD s SECTOR Imager to detect signals from microplate wells The kit includes a capture antibody for IL-4 and a ruthenium-labeled detection antibody It has a detection range of 0 05-1000 pg/mL with no cross-reactivity with other cytokines The kit provides a 96-well plate detection antibody calibrators 10X diluent and components for 96 tests
Supply Solutions Sigma Aldrich - ATP Bioluminescence Assay Kit CLS II * Third Party Item
Sigma Aldrich - ATP Bioluminescence Assay Kit CLS II * Third Party Item
NEOGEN β-Glucan Assay Kit, 100 assays, Enzymatic method, Absorbance detection, 510 nm wavelength, 4 to 100 μg range, 0.5 g/100 g detection limit, Total time ~100 min, Reagents stable > 2 years, Storage 2-8°C
β-Glucan Assay Kit, 100 assays, Enzymatic method, Absorbance detection, 510 nm wavelength, 4 to 100 μg range, 0.5 g/100 g detection limit, Total time ~100 min, Reagents stable > 2 years, Storage 2-8°C
Eurofins Discoverx PathHunter eXpress LTB4R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0292E2CP0M)
The PathHunter LTB4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. leukotriene B4) based activation of LTB4R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LTB4R and EA-tagged β-Arrestin-2. Activation of LTB4R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter GHSR Bioassay Kit, 2-Plate. (93-0242Y3-00141)
The PathHunter GHSR Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress TACR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0238E2CP0M)
The PathHunter TACR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. substance P) based activation of TACR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TACR2 and EA-tagged β-Arrestin-2. Activation of TACR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDG4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0255E2CP13M)
The PathHunter EDG4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. LPA) based activation of EDG4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG4 and EA-tagged β-Arrestin-2. Activation of EDG4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TOPOGEN INC DNA Gyrase Drug Screening Kit
This drug screening Kit is based on gyrase supercoiling action on a relaxed plasmid DNA substrate provided (pHOT1, which is a derivative of pBR322) and roughly 2.7 KB. One unit of gyrase will supercoil 200-500 ng in 1 hr at 37oC. The enzyme introduces negative supercoils into a relaxed pHOT1 plasmid substrate via a sign inversion model. An energy co-factor (ATP) is required for the complete supercoiling reaction. Non-EB gels are ideal for detecting gyrase activity as attested by the excellent resolution between supercoiled and relaxed DNA forms. It is designed to identify IFP and CIC (poisons and inhibitors, respectively). A typical IFP (e.g., a fluoroquinolone) will induce double strand DNA breaks while a prototypical CIC will simply block the enyzme from acting on relaxed DNA (novobiocin, some intercalators for example). Thus, an IFP (ciprofloxacin) will result in formation of a linear DNA product while a CIC will block supercoiling activity.
Eurofins Discoverx PathHunter eXpress HRH1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0503E2CP2L)
The PathHunter HRH1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH1 and EA-tagged β-Arrestin-2. Activation of HRH1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.