General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress CCR6 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0069E2CP2M)
The cAMP Hunter CCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20) based activation of CCR6 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR6, a naturally Gi/Go coupled GPCR. Upon activation of CCR6, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter LHCGR Bioassay Kit, 10-Plate. (95-0106Y2-00138)
The cAMP Hunter CHO-K1 LHCGR Bioassay Kit provides a robust, and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies.. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter ProLabel Detection Kit, 10,000 dp (384-well). (93-0180L)
The PathHunter ProLabel Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 25 plates (384-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0180l.
BioVendor, LLC QuickZyme Sensitive Tissue Collagen Assay Kit 96-Assays
QuickZyme Sensitive Tissue Collagen Assay Kit, 96-Assays. Measure hydroxyproline in small tissue samples with low collagen levels! The ONLY hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate, enabling measurement in small samples with low hydroxyproline levels, such as liver. No drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Dynamic range: 6-300 ug/ml. Sensitivity: 2.5 ug/ml. Sample vol. 50-500 ul. Species independent. Measures total hydroxyproline. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all collagen types present without discriminating between types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Collagen hydrolysis tubes & protocol ARE included. Ease-of-use: Equivalent to ELISA.
Molecular Devices Fluorescent Assay Kit, cGMP, 96-Well, Competitive Immunoassay
The CatchPoint cGMP Fluorescent Assay Kit is a 96-well competitive immunoassay for quantitative measurement of 3',5'-cyclic guanosine monophosphate (cGMP) and guanylate cyclase activity in biochemical and cell-based assays. The kit provides sensitive fluorescence-based detection and includes pre-coated microplates and optimized reagents for high-throughput workflows.
- 96-well format with a black microplate and clear bottom.
- Includes reagents and buffers for assay setup and detection.
- Reagents sufficient for two 96-well plates (192 reactions at 120 μL).
- Fluorescence detection using Stoplight Red substrate, compatible with common plate readers.
- Typical sensitivity down to ~0.2 nM cGMP, dynamic range up to ~120 nM.
- Store kit components at 4°C; reconstituted working solutions stable for about three weeks at 4°C.
Abcam Asparaginase Activity Assay Kit
Asparaginase Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Electron Microscopy Sciences Flim Sleeve 135 mm/24 exp with Clear open flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Clear open flap
Eurofins Discoverx InCELL Hunter eXpress BLK Kinase Binding Assay, 10pl Kit. (96-0044E1CP0L)
The InCELL Hunter eXpress BLK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BLK via detection of BLK levels. This product uses EFC technology. The cell line included overexpresses BLK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human Total AKT Detection Kit, 500 Assay Points
HTRF Human Total AKT Detection Kit, 500 Assay Points
Eurofins Discoverx cAMP Hunter™ Rat DRD1 Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Rat DRD1 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure rat DRD1 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ABclonal Technology HRH2 Rabbit pAb
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Eurofins Discoverx PathHunter eXpress C3AR1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0814E3CP0L)
The PathHunter C3AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C3a) based activation of C3AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C3AR1 and EA-tagged β-Arrestin-2. Activation of C3AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis - Economy kit (E. coli CFPS): 100 mL
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification. The 1 mL kit enables user to run 50 reactions of 20 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C. For research use only.
Eurofins Discoverx cAMP Hunter eXpress MTNR1A CHO-K1 GPCR Assay, 2-Plate Kit. (95-0086E2CP2M)
The cAMP Hunter MTNR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melatonin) based activation of MTNR1A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MTNR1A, a naturally Gi/Go coupled GPCR. Upon activation of MTNR1A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Lipid Assay Kit (unsaturated fatty acids)
Lipid Assay Kit (unsaturated fatty acids)
The product is subject to the following: Abcam Restricted Use Statement