General Cell Based Assays
Eurofins Discoverx PathHunter IL6RA/6ST Bioassay Kit, 10-Plate. (93-1045Y3-00044)
The PathHunter IL6RA/IL6ST Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-6-induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6R target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Innovative Research Inc Hmn uPA Rcmb LMW 100ug
Human Urokinase (uPA) Recombinant Low Molecular Weight 100ug - Human Urokinase (uPA) Recombinant Low Molecular Weight from Innovative Research has been recombinantly produced in insect cells and purified using a combination of conventional and affinity chromatography. This is a frozen liquid buffered in 0.05M Sodium Acetate; 0.1M NaCl; 1mM EDTA; pH 5.0 and a purity of 95%. It is useful as a positive control for ELISA and western blot and and other applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis. Purified from auto-catalytic digestion of recombinant human urokinase produced in insect cells. Active two-chain low molecular weight human urokinase contains the proteinase domain and is separated from the amino terminal fragment by cleavage at the Lys135-Lys136 bond.
Eurofins Discoverx PathHunter Human CXCL11 (CXCR3) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human CXCL11 (CXCR3) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CXCR3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress GIPR CHO-K1 GPCR Assay, 2-Plate Kit. (95-0146E2CP2M)
The cAMP Hunter GIPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GIPR, a naturally Gs coupled GPCR. Upon activation of GIPR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress LGR4 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0516E2ACP1L)
The PathHunter LGR4 (GPR48) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR4 and EA-tagged β-Arrestin-2. Activation of LGR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2C Activated GPCR Internalization Assay, 10pl Kit. (93-0669E3CP27L)
The PathHunter HTR2C Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HTR2C via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged HTR2C. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting HTR2C-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HRH1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0503E2CP2L)
The PathHunter HRH1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH1 and EA-tagged β-Arrestin-2. Activation of HRH1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR31 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0406E2CP2L)
The PathHunter GPR31 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR31 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR31 and EA-tagged β-Arrestin-2. Activation of GPR31 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DRD1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0590E2CP2L)
The PathHunter DRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD1 and EA-tagged β-Arrestin-2. Activation of DRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0083E2CP2L)
The cAMP Hunter DRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD1, a naturally Gs coupled GPCR. Upon activation of DRD1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human S100B ASY
The Meso Scale Discovery R-PLEX Human S100B Assay QuickPlex 5-PL Kit is a multiplex immunoassay for measuring S100B protein in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and rapid results The kit includes a plate with capture antibodies detection antibodies calibrators controls diluent read buffer wash buffer and adhesive seals for one 96-well plate It quantifies S100B levels from 0 04 ng/mL to 1 000 ng/mL requires 50 L of sample and is compatible with Meso Scale Discovery SECTOR Imagers
Eurofins Discoverx PathHunter eXpress OPRL1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0264E2CP0L)
The PathHunter OPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orphanin FQ) based activation of OPRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRL1 and EA-tagged β-Arrestin-2. Activation of OPRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AVPR1B CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0486E2CP0M)
The PathHunter AVPR1B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR1B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR1B and EA-tagged β-Arrestin-2. Activation of AVPR1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cINSRa Functional Assay, 2-Plate Kit. (93-1016E3CP16M)
The PathHunter eXpress cINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged cINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 13 Reagent, 100. (93-0563R13A)
AssayComplete Cell Plating 13 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.