General Cell Based Assays
Eurofins Discoverx PathHunter eXpress TrkB-P75 Functional Assay, 2-Plate Kit. (93-0530E3CP16M)
The PathHunter eXpress TrkB-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mADCYAP1R1 (PAC1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0594E2MCP0L)
The PathHunter mADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of mADCYAP1R1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mADCYAP1R1 and EA-tagged β-Arrestin-2. Activation of mADCYAP1R1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mSSTR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0616E2MCP2M)
The PathHunter mSSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of mSSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mSSTR2 and EA-tagged β-Arrestin-2. Activation of mSSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mGALR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0604E2MCP2L)
The PathHunter mGALR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of mGALR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGALR2 and EA-tagged β-Arrestin-2. Activation of mGALR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDNRB CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0593E2CP0L)
The PathHunter EDNRB eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-3) based activation of EDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRB and EA-tagged β-Arrestin-2. Activation of EDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TACR3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0501E2CP2M)
The PathHunter TACR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. substance P, senkitide) based activation of TACR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TACR3 and EA-tagged β-Arrestin-2. Activation of TACR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTHR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0306E2CP0M)
The PathHunter PTHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PTH) based activation of PTHR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR2 and EA-tagged β-Arrestin-2. Activation of PTHR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DRD5 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0426E2CP0M)
The PathHunter DRD5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD5 and EA-tagged β-Arrestin-2. Activation of DRD5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR3 CHO-K1 β-Arrestin GPCR Assays, 10-Plate Kit. (93-0271E2CP2L)
The PathHunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR3 and EA-tagged β-Arrestin-2. Activation of CXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EGFR/EGFR Dimerization Assay, 2-Plate Kit. (93-0989E3CP5M)
The PathHunter EGFR/EGFR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. EGF) induced dimerization of EGFR/EGFR receptors. This product uses EFC technology. The cell line included overexpresses EGFR target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of EGFR/EGFR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc AlphaLISA High Performance Biotin-Free Human TNFα Detection Kit, 100 Assay Points
AlphaLISA High Performance Biotin-Free Human TNFα Detection Kit, 100 Assay Points
Montana Molecular Green Upward cADDis cAMP sensor Big Sky BacMam 5mL
Detect cAMP signaling kinetics in living cells with the Green Upward cADDis fluorescent cAMP assay. Increase in fluorescence indicates increase in cAMP. Packaged in Big Sky BacMam, for enhanced expression in HEKs, CHOs, and many other mammalian cell types and iPSCs. This is an updated version of U0200G, with improved expression efficiency from Big Sky BacMam.
Molecular Devices Membrane Potential Assay Kit, Red, Explorer, Homogeneous Fluorescence
The FLIPR Membrane Potential Assay Kit (Red, Explorer) is a homogeneous, fluorescence-based reagent kit for sensitive detection of rapid changes in cellular membrane potential. It is optimized for high-throughput screening and fast kinetic measurements and is compatible with automated plate readers and FLIPR/Tetra systems.
- Homogeneous fluorescence formulation for rapid membrane potential detection.
- Red indicator optimized for larger signal window and temperature stability.
- Explorer format supplies reagents for approximately 10-15 microplates.
- Compatible with 96-, 384-, and 1536-well plate formats.
- Suitable for ion channel and transporter assays, including TRP and voltage-gated channels.
- Free of animal-derived components.
Molecular Devices cAMP Fluorescent Assay Kit, 384-Well, Bulk, 60 μL
The CatchPoint cAMP Fluorescent Assay Kit is a high-throughput competitive immunoassay for quantifying 3',5'-cyclic adenosine monophosphate (cAMP) and measuring adenylate cyclase activity in biochemical and cell-based assays. The 384-well bulk format supplies reagents for 20 plates (7,680 assay points) and is optimized for fluorescence intensity detection with long signal stability.
- 384-well bulk format, sufficient for 20 plates (7,680 wells)
- Reaction volume 60 μL per well, 50 μL supported for some HTS systems
- Fluorescence intensity detection using Stoplight Red substrate
- Minimum detectable concentration 0.1 nM (6 fmol/well), EC50 approximately 3.4 nM
- Readings stable from 10 minutes up to 24 hours after substrate addition
- Store components at 4°C; kit components stable for six months when stored properly
Eurofins Discoverx PathHunter eXpress rOPRM1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0494E2RCP2L)
The PathHunter rOPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE) based activation of rOPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rOPRM1 and EA-tagged β-Arrestin-2. Activation of rOPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.