General Cell Based Assays
Cell Biolabs Inc Total Cholesterol Assay Kit (Fluorometric), 192 assays
Cell Biolabs’ Total Cholesterol Assay Kit is a simple fluorometric assay that measures the amount of total cholesterol present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, cholesterol standards and unknown samples. Sample cholesterol concentrations are determined by comparison with a known cholesterol standard. Cholesteryl esters can be quantified by subtracting the free cholesterol values from the total cholesterol value.
Eurofins Discoverx PathHunter Human YES1 Kinase Activity Cell-Based Assay Kit (U2OS)
The PathHunter Human YES1 Stable Cell Line Kinase Activity Assay (U2OS) measures YES1 activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ABclonal Technology GCGR Rabbit pAb
The protein encoded by this gene is a glucagon receptor that is important in controlling blood glucose levels. Defects in this gene are a cause of non-insulin-dependent diabetes mellitus (NIDDM).
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD4(1) Bromodomain Assay, 10-plate Kit. (96-0005E1CP0L)
The InCELL Hunter eXpress HEK 293 BRD4(1) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD4(1) via detection of BRD4(1) levels. This product uses EFC technology. The cell line included overexpresses BRD4(1) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter™ Human TSHR(L) Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human TSHR(L) Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure TSHRL (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Sigma Aldrich Fine Chemicals Biosciences fluorometric Intracellular
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels ROS contribute to cell signaling and host defense. Increased ROS generation above the detoxification capacity of the biological system results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids essential proteins and DNA. ROS has been implicated in disease states such as Alzheimers disease Parkinsons disease cancer and aging.
Eurofins Discoverx PathHunter eXpress CHRM2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0198E2CP0L)
The PathHunter CHRM2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM2 and EA-tagged β-Arrestin-2. Activation of CHRM2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GPR123 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0417E2ACP1M)
The PathHunter GPR123 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR123 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR123 and EA-tagged β-Arrestin-2. Activation of GPR123 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GHRHR CHO-K1 GPCR Assay, 10-Plate Kit. (95-0076E2CP2L)
The cAMP Hunter GHRHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GRF) based activation of GHRHR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GHRHR, a naturally Gs coupled GPCR. Upon activation of GHRHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Total Histone H3 Detection Kit, 500 Assay Points
HTRF Total Histone H3 Detection Kit, 500 Assay Points
Meso Scale Discovery U-PLEX Human MCP-1 ASY 25 PL
The Meso Scale Discovery U-PLEX Human MCP-1 Assay Kit quantifies monocyte chemoattractant protein 1 (MCP-1) in human serum plasma and cell culture supernatants Using MSD s SULFO-TAG labels and multi-array technology it measures MCP-1 levels down to 0 4 pg/mL The kit includes a 96-well plate with capture antibodies detection antibodies buffers blockers calibrators and controls The assay takes about 3 5 hours and requires an MSD instrument like the MESO QuickPlex SQ 120 It offers high sensitivity broad range and minimal sample volume for efficient MCP-1 analysis
ABclonal Technology TrKC Rabbit pAb
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene.
TRIBIOSCIENCE INC Mitochondrial Complex III Activity Colorimetric Assay 100 Tests
The activity of Complex III is directly measured through the increase of reduction of cytochrome c at absorbance of 550nm. This kit uses cytochrome c as substrate. The reduced cytochrome c is determined by recording the change in absorbance (A550). Applications Complex III Activity.
Cayman Chemical Glycogen FluorometrIc Detecto
A GnRH receptor antagonist (Kis 6 2.2 and 3.8 nM for the human mouse and rat receptors respectively) selective for the GnRH receptor over a panel of 43 receptors ion channels and enzymes at 1 UM prevents GnRH-induced increases in inositol phosphate production in HEK293 cells expressing the human GnRH receptor (KB 25 nM) oral or intravenous administration decreases plasma LH levels in castrated rats inhibits GnRH-A-induced increases in plasma LH and testosterone levels in non-castrated rats at 20 mg/kg