General Cell Based Assays
Genscript Corporation CHO-K1/Ga15/AMY3 Stable Cell
Receptor activity-modifying proteins (RAMPs) are a class of protein which interact with and modulate the activities of several Class B G Protein-Coupled Receptors including the receptors for secretin calcitonin (CT) glucagon and vasoactive intestinal peptide (VIP) There are three distinct types of RAMPs designated RAMP1 RAMP2 and RAMP3 each encoded by a separate gene Currently the function of RAMPs is divided into 2 class activities Association of RAMPs with either the CT or CALCRL proteins forms 6 different receptors from the calcitonin receptor family When associated with the Calcitonin receptor (CTR) or Calcitonin receptor-like (CALCRL) RAMPs can change the selectivity of the receptor for a specific hormone In the cases of the other receptors mentioned however there is no evidence that they can do this but instead function to regulate trafficking of receptors from the ER / golgi to the membrane GenScript s cloned human AMY3 expressing cell line is generated in the C
Eurofins Discoverx PathHunter eXpress CCR5 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0224E2CP0L)
The PathHunter CCR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL3/MIP-1α) based activation of CCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR5 and EA-tagged β-Arrestin-2. Activation of CCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human CD80/B7-1 ASY
The R-PLEX Human CD80 (B7-1) Assay Kit from Meso Scale Discovery measures CD80 levels in human serum plasma and cell culture supernatants using electrochemiluminescence (ECL) detection It offers high sensitivity broad dynamic range and precision The kit includes a 96-well plate with capture and detection antibodies buffers blocker solutions calibrator and controls Sensitivity is 0 3 pg/mL with an upper quantification limit of 10 000 pg/mL suitable for biomarker discovery and immunology research
Meso Scale Discovery U-PLEX Mouse IL-2 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-2 Assay Kit is a sensitive immunoassay for detecting and quantifying mouse interleukin 2 (IL-2) in various biological fluids It uses MSD s electrochemiluminescent detection technology and includes plates with capture antibodies detection antibodies and calibrators The assay has a sensitivity of 0 06 pg/mL a dynamic range over 3 logs requires 50 L sample volume and is compatible with MSD platforms The kit contains all necessary reagents for measuring mouse IL-2
Meso Scale Discovery RPLEX HUM IL28BIFN 3 ASY
The R-PLEX Human IL-28B (IFN-lambda3) Assay is a multiplex immunoassay kit from Meso Scale Diagnostics that enables the quantitative measurement of human interleukin 28B (IL-28B) also known as interferon lambda 3 (IFN-lambda3) This assay utilizes MSD s electrochemiluminescent detection technology and is provided in a 96-well plate format The assay has a detection range of 0 03-1000 pg/mL and requires 50 L of sample per well This product can be used to measure IL-28B levels in serum plasma and cell culture supernatants IL-28B is a cytokine that has antiviral activity and is involved in immune response against viruses
Meso Scale Discovery R-PLEX Rat IL-6 ASY
The Meso Scale Discovery R-PLEX Rat IL-6 Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of rat interleukin 6 (IL-6) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision with low sample volume requirements The assay requires 50 L or less of sample and has a testing time of 3 5 hours The lower limit of detection for rat IL-6 is 1 3 pg/mL This assay is for research use only and is not intended for diagnostic procedures It is available as a singleplex assay or as part of a multiplex panel
Eurofins Discoverx PathHunter eXpress OPN5 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0704E2CP1L)
The PathHunter OPN5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of OPN5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPN5 and EA-tagged β-Arrestin-2. Activation of OPN5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR3 CHO-K1 β-Arrestin GPCR Assays, 2-Plate Kit. (93-0271E2CP2M)
The PathHunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR3 and EA-tagged β-Arrestin-2. Activation of CXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human CXCL11 (CXCR3) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human CXCL11 (CXCR3) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure CXCR3 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Sino Biological Human IL-1RAcP / IL-1R3 Protein (His Tag), Endotoxin-Free 100µg
A DNA sequence encoding the human IL1R3 (NP_002173.1) extracellular domain (Met 1-Glu 359) was expressed, fused with the a C-terminal polyhistidine tag.
Cayman Chemical CellBased Assay Buffer Tablet
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Medchemexpress LLC GNE-987 GSH linker-1 | 2417370-39-7 | C62H77F2N9O12S4 | 10MG
GNE-987 GSH linker-1 is a PROTAC-linker conjugate that pairs a BET-degrading warhead with a disulfide-containing glutathione-cleavable linker for use in PROTAC-antibody conjugate (PAC) research. It is intended as a research reagent for chemical biology studies involving targeted protein degradation and intracellular payload release.
- Enables targeted protein degradation when conjugated to antibodies.
- Contains a disulfide-based glutathione-cleavable linker for intracellular release.
- High chemical purity suitable for analytical and biological testing.
- Compatible with common solvents and recommended storage protocols.
- Applicable to studies of protein degradation pathways and PAC development.
Meso Scale Discovery R-PLEX Human B2M ASY
The R-PLEX Human B2M Assay from Meso Scale Diagnostics is a multiplex immunoassay kit for measuring beta-2 microglobulin (B2M) in human serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection for high sensitivity and precision Requiring 50 L or less sample volume it performs up to 80 tests on two plates The kit includes a 96-well plate with capture antibody detection antibody buffers diluent and calibrators The assay time is 3 5 hours with a calibration range of 50-10 000 pg/mL
Eurofins Discoverx PathHunter Human OXER1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human OXER1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure OXER1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TORC3 (CRTC3) Nuclear Translocation Cell-Based Assay Kit (U2OS)
The PathHunter Human TORC3 (CRTC3) Stable Cell Line Nuclear Translocation Assay (U2OS) contains a stable clonal cell line used to measure CRTC/CREB pathway activation via nuclear translocation of TORC3. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.