General Cell Based Assays
Ethos Biosciences Exocell DTNB Thiol Oxidative Stress Assay, Ethos
The Ethos Biosciences DTNB (Ellman’s) Thiol Oxidative Stress Assay Kit is designed to measure free thiol groups that arise due to oxidative stress, which is an underlying component of acute and chronic kidney disease. Our kit uses Ellman’s Reagent otherwise known as DTNB or 5-5′-dithiobis [2-nitrobenzoic acid] which reacts with the disulfide bond in a sample to produce 2-nitro-5-thiobenzoic acid. Under conditions of oxidative stress, free thiols decrease and disulfides increase. Determination of the free thiol concentration reflects the ability to detoxify lipid (and other) peroxides. Aliquots of standard or sample are reacted with rehydrated Ellman’s reagent (DTNB) in microtiter wells. Our DTNB (Ellman’s) Thiol Oxidative Stress Assay kit contains all of the components and reagents you need to conduct a 96 well plate chemical assay for thiol groups of any species in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…)
Eagle Biosciences Inc Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
TRIBIOSCIENCE INC Hexokinase Activity Colorimetric Assay, 100 Tests
Tribioscience’s Hexokinase Activity Colorimetric Assay kit provides a flexible, accurate, sensitive, and time-saving approach for detecting HK activity in a wide variety of samples. In this assay, HK converts glucose into glucose-6-phosphate (G6P), then undergoes a series of reactions and induces the Colorimetric-Probe to generate a strong colored product detected at 460 nm.
Revvity Health Sciences Inc HTRF HMGB1 Detection Kit, 500 Assay Points
HTRF HMGB1 Detection Kit, 500 Assay Points
Abcam Hexokinase Activity Assay Kit (Colorimetric)
Hexokinase Activity Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Uricase Assay Kit (Fluorometric)
Uricase Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Phalloidin-iFluor 555 Reagent
Phalloidin-iFluor 555 Reagent
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx cAMP Hunter Liraglutide Bioassay Kit, 2-Plate. (95-0062Y2-00099)
The cAMP Hunter Liraglutide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Liraglutide based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GPR3 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0626E2CP1M)
The PathHunter GPR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR3 and EA-tagged β-Arrestin-2. Activation of GPR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.