General Cell Based Assays
Eurofins Discoverx cAMP Hunter™ Human MTNR1A Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human MTNR1A Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure MTNR1A (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress GPR35 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0152E2CP2M)
The cAMP Hunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR35, a naturally Gi/Go coupled GPCR. Upon activation of GPR35, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Superoxide Dismutase SOD A
Superoxide dismutase (SOD) is part of a family of metal ion-containing enzymes (SODs) that are found in most organisms from bacteria to mammals. SODs catalyze the dismutation of superoxide (generated by aerobic respiration) to molecular oxygen and peroxide. Superoxide dismutase form the front line of defence against reactive oxygen species (ROS)-mediated injury and harbor anti-inflammatory activities. The Superoxide Dismutase (SOD) Activity Assay Kit provides a simple and sensitive procedure for measuring SOD enzymatic activity in various sample types. SOD activity is determined by measuring the decrease in superoxide anions (generated by the enzyme xanthine oxidase [XO]). Since superoxide anions interact with the provided WST dye yielding color at 450 nm the decrease in the color signal is proportional to SOD inhibition activity. The kit allows the determination of SOD activity in percent inhibition or in units of activity using the provided SOD Enzyme as a standard. The provided
LICORbio GAR CellTag™ 700 Stain ICW Kit II
GAR CellTag 700 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 700 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. This kit includes reagents to perform an In-Cell Western assay and CellTag 700 Stain to normalize well-to-well variations in cell number. CellTag 700 accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. Kit Components include IRDye 800CW Goat anti-Rabbit Secondary Antibody (0.5 mg), Intercept (TBS) Blocking Buffer (1 x 500 mL), CellTag 700 Stain (2 x 10 nmol), and Kit Protocol
Eurofins Discoverx PathHunter eXpress HCRTR1 Total GPCR Internalization Assay, 10pl Kit. (93-0729E3CP0L)
The PathHunter HCRTR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Total GSK3β Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total GSK3β Detection Kit, 50,000 Assay Points
Abcam Acetylcholinesterase Assay Kit (Fluorometric Red)
Acetylcholinesterase Assay Kit (Fluorometric Red)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Comet Assay Kit (3-well slides)
Comet Assay Kit (3-well slides)
The product is subject to the following: Abcam Restricted Use Statement
Miltenyi Biotec, Inc. IFN-g Secr Assay - Enr&Det Kit (PE), h
Isolation and detection of human IFN-γ–secreting cells.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Eurofins Discoverx PathHunter eXpress IL23R/IL12RB1 Dimerization Assay, 10pl Kit. (93-1007E3CP0L)
The PathHunter IL23R/IL12RB1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-23) induced dimerization of IL23R/IL12RB1 receptors. This product uses EFC technology. The cell line included overexpresses IL23R target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL23R/IL12RB1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 CHO-K1 β-Arrestin-1 GPCR Assay, 2pl Kit. (93-1050E2CP2M)
The PathHunter AGTRL1 (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-1. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam SREBP-2 Transcription Factor Assay Kit.
SREBP-2 Transcription Factor Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement