General Cell Based Assays
Eurofins Discoverx InCELL Hunter eXpress BTK Kinase Binding Assay, 2pl Kit. (96-0056E15CP0M)
The InCELL Hunter eXpress BTK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BTK via detection of BTK levels. This product uses EFC technology. The cell line included overexpresses BTK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress LHCGR CHO-K1 GPCR Assay, 10-Plate Kit. (95-0106E2CP2L)
The cAMP Hunter LHCGR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. luteinizing hormone) based activation of LHCGR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type LHCGR, a naturally Gs coupled GPCR. Upon activation of LHCGR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric).
Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric).
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress PTHR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0315E2CP0M)
The PathHunter PTHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PTH) based activation of PTHR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR1 and EA-tagged β-Arrestin-2. Activation of PTHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress AVPR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0150E2CP2L)
The cAMP Hunter AVPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type AVPR2, a naturally Gs coupled GPCR. Upon activation of AVPR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc AlphaScreen HA Detection Kit, 10,000 Assay Points
AlphaScreen HA Detection Kit, 10,000 Assay Points
Cayman Chemical α-D-Glucose-1-phosphate (sodium salt hydrate), Purity: ≥95%, CAS: 150399-99-8, 5 g
An intermediate in glycogen metabolism; precursor in the biosynthesis of UDP-glucose; can be formed during glycogen breakdown via phosphorolytic cleavage of glycogen by glycogen phosphorylase; can be converted to glucose-6-phosphate by phosphoglucomutase; is combined with CTP to form CDP-glucose in S. typhi
Eurofins Discoverx PathHunter Human OPRL1 (Opioid) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human OPRL1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure OPRL1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human DRD2S β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human DRD2S β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure DRD2S (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences ApoSENSOR™ Cell viability assay kit (200 tests)
The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well). Applications: Chemiluminescence .
CPC Scientific H-Thr-Ser-Gly-Pro-Leu-Gly-Asn-Leu-Ala-Glu-Glu-Leu-Asn-Gly-Tyr-Ser-Arg-Lys-Lys-Gly-Gly-Phe-Ser-Phe-Arg-Phe-NH2 (trifluoroacetate salt) 0.5MG
An RF-amide peptide ligand for SP9155, an orphan G-protein-coupled receptor with high homology to orexin, neuropeptide FF and CCK receptors. It has been shown to potently activate SP9155 with an EC50 of 7 nM.ONE-LETTER SEQUENCE: TSGPLGNLAEELNGYSRKKGGFSFRF-NH2MOLECULAR FORMULA: C127H195N37O37MOLECULAR WEIGHT:2832.17STORAGE CONDITIONS: -20 5C, CAS REGISTRY NUMBER: [600171-68-4], RESEARCH AREA: PainREFERENCES: N.Chartrel et al., Proc. Natl. Acad. Sci. USA, 100, 15247 (2003); Y.Jiang et al., J. Biol. Chem., 278, 27652 (2003)
Abcam Tyrosinase Activity Assay Kit (Colorimetric)
Tyrosinase Activity Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Bioassay Systems EnzyChrom™ Acetate Assay Kit. For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.
For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism. Key Features: Sensitive. Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays. Simple. The assay involves adding a single working reagent, incubation for 30 min at room temperature and read. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, food, agriculture and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.13 mM.
Eurofins Discoverx PathHunter eXpress mGLP1R CHO-K1 β-Arrestin-1 GPCR Assay, 2pl Kit. (93-0974E2MCP2M)
The PathHunter mGLP1R (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGLP1R and EA-tagged β-Arrestin-1. Activation of mGLP1R stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.