General Cell Based Assays
Cayman Chemical L-Lactate asy KIt 96Wel
Cayman’s L-Lactate Assay provides both a fluorescence and an absorbance-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, LDH catalyzes the oxidation of lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the substrate to yield a final product that can be measured fluorometrically with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm, or colorimetrically at 535 nm.
Cayman Chemical ThIol DetectIon asy KIt 96Wel
The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol Detection provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilizes a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380-390 nm and emission wavelengths between 510-520 nm.
Cayman Chemical AconItase asy KIt 96Wel
Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. Cayman’s Aconitase Assay provides a simple, reproducible, and sensitive tool for assaying aconitase from tissue homogenates or cell lysates.
Cayman Chemical CholesTRol Uptake CelBased 1ea
Maintaining body cholesterol homeostasis is critical for normal physiological functions and is achieved by a highly regulated balance of de novo synthesis, dietary cholesterol absorption, biliary clearance, and excretion. The mechanisms controlling cholesterol absorption are important to understand for the prevention and treatment of hypercholesterolemia. Cayman’s Cholesterol Uptake Cell-Based Assay Kit provides a convenient tool for studying cholesterol absorption modulators. The kit employs NBD Cholesterol, a fluorescently tagged cholesterol, as a probe for the detection of cholesterol taken up by cultured cells. U-18666A, a cholesterol intracellular trafficking inhibitor, is included as a positive control.
Cayman Chemical D-Lactate Stndard 1ea
A clear uncoated flat-bottom 96 well plate for use in colorimetric assays.
Cayman Chemical GPX4 GlutathIon Reductase 1ea
GPX4 Glutathione Reductase has been tested and formulated to work exclusively with Cayman’s GPX4 Inhibitor Screening Assay Kit (Item No. 701880).
Cayman Chemical Ethnol asy Reagent 2mL
A thymoleptic and tricyclic antidepressant inhibits 5-HT uptake in rat brain synaptosomes (IC50 670 nM) increases the lethality of yohimbine in mice at 20 and 40 mg/kg
Cayman Chemical Hydrogen PRoxID asy KIt 480Wel
An analytical reference standard categorized as a lysergamide intended for research and forensic applications
Cayman Chemical SupRoxID DIsmutase asy KI 480
Cayman’s Superoxide Dismutase Assay Kit provides a convenient method of measuring SOD activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates. The assay relies on xanthine oxidase to produce superoxide radicals, which then reduce a tetrazolium salt to a formazan dye, detectable at 450 nm. SOD in the sample inhibits this reduction by catalyzing the dismutation of superoxide radicals. Thus, the degree of inhibition is directly proportional to SOD activity. The SOD Assay Kit measures all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Mitochondrial Mn-SOD can be assayed separately following the procedure outlined in the kit booklet.
Cayman Chemical Cel-Based asy VerapamIl 50uL
A polyamine bile amidate has been found in lion but not mouse fecal samples has been used as a standard in the detection of chenodeoxycholyl-putrescine in mammalian intestinal and fecal samples
Cayman Chemical DTNB asy Reagent 1ea
An inhibitor of GAC inhibits GAC enzyme activity (EC50 10.64 nM) reduces the viability of A549 NSCLC cells (IC50 4.025 nM) reduces colony formation by and intracellular glutamate levels in A549 cells at 0.1 and 1 UM reduces tumor growth in an A549 mouse xenograft model at 100 mg/kg
Cayman Chemical LDH CytotoxIcIty asy KIt 480
For measurement of LDH activity as an indicator of cell death
Cayman Chemical EstradIol ELISA KIt 96solId
Cayman’s Estradiol ELISA Kit is a competitive assay that can be used for the quantification of estradiol in plasma and serum. This assay has a range of 0.61-10,000 pg/ml with a sensitivity of approximately 20 pg/ml (80% B/B0)
Cayman Chemical Catlase asy KIt 96Wel
Catalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS production. Cayman’s Catalase Assay utilizes the peroxidatic function of catalase for determination of enzyme activity. The assay can be used to measure catalase activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates.
Cayman Chemical NADNADH Cel-Based asy KIt 1ea
Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric ethod for measuring intracellular NAD+and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.