General Cell Based Assays
Meso Scale Discovery U-PLEX Human CA1 ASY 25 PL
The Meso Scale Discovery U-PLEX Human CA1 Assay is a multiplex immunoassay kit for measuring CA1 in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and rapid results Requiring 50 L or less sample volume it is compatible with Meso Scale Discovery platforms The kit includes a 96-well plate with capture and detection antibodies plus calibrators and controls for standard curve generation and assay validation offering a simple sensitive and reproducible method to measure CA1 levels
Cayman Chemical MItrOS580 500test
A fluorescent probe for mitochondrial superoxide production in live cells; cell-permeable and selectively targets mitochondria; upon oxidation by superoxide, MitoROS™ 580 fluoresces, and this fluorescence can be measured using fluorescence microscopy or flow cytometry; ex/em = 510/580 nm; has been used to determine effects of curcumin treatment on superoxide production in Jurkat cells
Revvity Health Sciences Inc HTRF Human & Mouse TOM20 Detection Kit, 500 Assay Points
HTRF Human & Mouse TOM20 Detection Kit, 500 Assay Points
Cayman Chemical RXR RetInoId X receptr100uL
A KCC2 cotransporter activator (EC50 616 nM for chloride transport in NG108-cl cells) selective for KCC2 over GABAA receptors at 50 UM increases the rate of chloride accumulation in spinal slices isolated from a rat model of PNI and BDNF control slices increases mechanical withdrawal thresholds in a rat model of PNI and reverses morphine-induced hyperalgesia in rats at 100 mg/kg
Cayman Chemical Pyruvate asy Cofactr MIxtu 1ea
An anticancer polyamine inhibits the proliferation of PC3 TSU-pr1 DU145 and JCA-1 cells (IC50s 65 62 3 and 21 UM respectively) reduces ODC activity and increases diamine acetyltransferase 1 activity in PC3 DU145 and JCA-1 cells at 100 UM increases H2O2 levels and disrupts the mitochondrial membrane potential in U87 and LN-229 cells at 10 UM decreases tumor growth in a DU145 mouse xenograft model at 40 mg/kg three times per day
ABclonal Technology LDH-B
This gene encodes the B subunit of lactate dehydrogenase enzyme which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD in a post-glycolysis process Alternatively spliced transcript variants have been found for this gene Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism and that this isoform is localized in the peroxisomes Mutations in this gene are associated with lactate dehydrogenase B deficiency Pseudogenes have been identified on chromosomes X 5 and 13
Eurofins Discoverx InCELL Hunter eXpress BTK Kinase Binding Assay, 2pl Kit. (96-0056E15CP0M)
The InCELL Hunter eXpress BTK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BTK via detection of BTK levels. This product uses EFC technology. The cell line included overexpresses BTK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress A549 G9a Methyltransferase Assay, 10-plate Kit. (96-0003E15CP7L)
The InCELL Hunter eXpress A549 G9a Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of G9a via detection of G9a levels. This product uses EFC technology. The cell line included overexpresses G9a target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.