General Cell Based Assays
Eurofins Discoverx PathHunter eXpress NMU1R U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0232E3CP0M)
The PathHunter nMU1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuromedin U) based activation of nMU1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged nMU1R and EA-tagged β-Arrestin-2. Activation of nMU1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 27 Reagent, 500. (93-0563R27B)
AssayComplete Cell Plating 27 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress mCCR7 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0528PE2MCP0M)
The PathHunter mCCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of mCCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR7 and EA-tagged β-Arrestin-2. Activation of mCCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse IL-22 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-22 Assay Kit is a multiplex immunoassay for measuring mouse interleukin 22 (IL-22) in various samples It uses electrochemiluminescence detection for high sensitivity and precision The kit includes a plate with capture antibodies ECL-tagged detection antibodies calibrators controls and necessary buffers It requires 50 L of sample per well and quantifies IL-22 from 1 5 to 10 000 pg/mL in about 3 5 hours aiding in the study of mucosal immunity tissue regeneration and inflammation
Eurofins Discoverx PathHunter eXpress IL7R/IL2RG Dimerization Assay, 10pl Kit. (93-0997E13CP0L)
The PathHunter IL7R/IL2RG Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-7) induced dimerization of IL7R/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside IL2RG target protein fused to EA. Upon ligand-induced dimerization of IL7R/IL2RG, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCX3CR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0702E2MCP2M)
The PathHunter mCX3CR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CX3CL1, fractalkine) based activation of mCX3CR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCX3CR1 and EA-tagged β-Arrestin-2. Activation of mCX3CR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress C5AR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0079E2CP2L)
The cAMP Hunter C5AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type C5AR1, a naturally Gi/Go coupled GPCR. Upon activation of C5AR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM5 Activated GPCR Internalization Assay, 2pl Kit. (93-0657E3CP5M)
The PathHunter CHRM5 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress FGFR4-β-Klotho Functional Assay, 10pl Kit. (93-1060E3CP0L)
The PathHunter eXpress FGFR4-β-Klotho Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR4 and β-Klotho dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, β-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MCHR2 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0691E3CP5M)
The PathHunter MCHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MCH) based activation of MCHR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MCHR2 and EA-tagged β-Arrestin-2. Activation of MCHR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0859E3CP0M)
The PathHunter CHRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM1 and EA-tagged β-Arrestin-2. Activation of CHRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCCR9 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0734E2MCP2M)
The PathHunter mCCR9 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL25) based activation of mCCR9 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR9 and EA-tagged β-Arrestin-2. Activation of mCCR9 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Follitropin Alfa Bioassay Kit, 2pl. (95-0119Y2-00103)
The cAMP Hunter Follitropin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Follitropin Alfa based activation of FSHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FSHR, a naturally Gs coupled GPCR. Upon activation of FSHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress PTGIR Total Internalization Assay, 10pl Kit. (93-0838E3CP0L)
The PathHunter PTGIR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGIR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGIR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGIR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems EnzyChrom™ Glucose Assay Kit III. For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 0.03 to 2 mM (0.54 mg/dL to 36 mg/dL) glucose. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.03 mM.
