General Cell Based Assays
Meso Scale Discovery U-PLEX Human Nectin-4 ASY
The Meso Scale Discovery Human Nectin-4 Singleplex Assay Kit is a singleplex immunoassay that measures Nectin-4 levels in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity wide dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The assay has a typical lower limit of detection of 0 03 ng/mL and an upper limit of quantification of 2500 ng/mL This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator and control
Meso Scale Discovery R-PLEX Human B2M ASY
The R-PLEX Human B2M Assay from Meso Scale Diagnostics is a multiplex immunoassay kit for measuring beta-2 microglobulin (B2M) in human serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection for high sensitivity and precision Requiring 50 L or less sample volume it performs up to 80 tests on two plates The kit includes a 96-well plate with capture antibody detection antibody buffers diluent and calibrators The assay time is 3 5 hours with a calibration range of 50-10 000 pg/mL
Cayman Chemical nxIn V APC asy KIt 1ea
Cayman's Annexin V APC Assay Kit employs an APC-conjugated annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. DAPI is used as a marker of cell membrane permeability seen in very late apoptotic or necrotic cells. This kit is valuable for researchers looking to assess the degree of apoptosis induced by experimental compounds, and can be useful in pharmacology, immunology, cancer, or physiology research.
Meso Scale Discovery R-PLEX AB40 4G8 ASY
The Meso Scale Discovery R-PLEX Human Abeta40 4G8 Assay Kit is a multiplex immunoassay that measures Abeta40 levels in human samples such as serum plasma CSF and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The kit includes a plate coated with a 4G8 antibody to capture Abeta40 along with detection antibodies calibrators controls buffers and other reagents needed to run the assay It allows rapid and accurate quantification of Abeta40 levels which may be useful as a biomarker in Alzheimer s disease research and drug development
Meso Scale Discovery U-PLEX Human G-CSF ASY
The Meso Scale Discovery U-PLEX Human G-CSF Assay is a multiplex immunoassay kit that allows simultaneous measurement of Granulocyte Colony Stimulating Factor (G-CSF) in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This assay requires 50 L or less sample volume and can be read on MSD instrument platforms It includes a plate coated with capture antibodies detection antibodies and calibrators and controls needed to generate a standard curve and measure G-CSF concentrations
Eurofins Discoverx PathHunter eXpress IL6RA/IL6ST Dimerization Assay, 2pl Kit. (93-1045E3CP5M)
The PathHunter IL6RA/IL6ST Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-6) induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6RA target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR6 Total Internalization Assay, 10pl Kit. (93-0886E3CP0L)
The PathHunter CXCR6 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR6 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CXCR6 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CXCR6 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0859E3CP0L)
The PathHunter CHRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM1 and EA-tagged β-Arrestin-2. Activation of CHRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress FGR Activity Assay, 2-Plate Kit. (93-0884E3CP16M)
The PathHunter eXpress FGR Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the FGR kinase domain. The cell line is engineered to express the catalytic domain from FGR fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the FGR catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRK1 Total Internalization Assay, 10pl Kit. (93-0828E3CP0L)
The PathHunter OPRK1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OPRK1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged OPRK1 and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRK1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress VIPR2 Total Internalization Assay, 2pl Kit. (93-0824E3CP5M)
The PathHunter VIPR2 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of VIPR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged VIPR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the VIPR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Phospho-IR-β (Insulin Receptor Beta) (Tyr1150 /1151) Detection Kit, 500 Assay Points
HTRF Phospho-IR-β (Insulin Receptor Beta) (Tyr1150 /1151) Detection Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress GLP1R Total GPCR Internalization Assay, 10pl Kit. (93-1075E3CP5L)
The PathHunter GLP1R Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GLP1R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged GLP1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GLP1R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL1RL1/IL1RAP Dimerization Assay, 10pl Kit. (93-1067E3CP5L)
The PathHunter IL1RL1/IL1RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-33) induced dimerization of IL1RL1/IL1RAP receptors. This product uses EFC technology. The cell line included overexpresses IL1RL1 target protein fused to PK alongside IL1RAP target protein fused to EA. Upon ligand-induced dimerization of IL1RL1/IL1RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.