General Cell Based Assays
Bioassay Systems EnzyFluo™ AMPK Phosphorylation Assay Kit. For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.
For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.
Electron Microscopy Sciences Flim Sleeve 135 mm/24 exp with Clear open flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Clear open flap
Eurofins Discoverx PathHunter eXpress mC5AR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0577E2MCP0L)
The PathHunter mC5AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of mC5AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mC5AR1 and EA-tagged β-Arrestin-2. Activation of mC5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0308E2CP2M)
The PathHunter SSTR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR4 and EA-tagged β-Arrestin-2. Activation of SSTR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Med Vet International Farrier Kit, J1024K, Each
Set of essential farrier equipment in an attractive black zipper case with carrying handles. • Hoof rasp with wooden handle • Farrier hammer • Standard hoof knife • Clinch cutter • Hoof nipper • Shoe puller • Weights 9.2 lbs • Measures 10" W x 1 3/4" D x 24" L
Eurofins Discoverx PathHunter eXpress CHRM5 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0519E2CP0M)
The PathHunter CHRM5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM5 and EA-tagged β-Arrestin-2. Activation of CHRM5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDNRB Total Internalization Assay, 2pl Kit. (93-0874E3CP0M)
The PathHunter EDNRB Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of EDNRB via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged EDNRB and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDNRB receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc Radius™ 48-Well Cell Migration Assay Kit, 48 assays
Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The Radius™ 48-Well Cell Migration Assay Kit uses a proprietary 48-well plate to monitor migration of cells. Each plate well contains a 0.68 mm non-toxic, biocompatible hydrogel spot where cells do not attach. Adherent cells are seeded and attach everywhere but the Radius™ Gel coated area. Once firm attachment is achieved, the hydrogel is quickly removed to expose a cell-free region across which cells can migrate. The kit provides qualitative or quantitive results with real-time or endpoint analysis, and it is compatible with most HCS and liquid handling instruments. Radius Cell Migration Assays are also available as well in 24-well, 96-well and 384-well formats, and Radius 24-Well Assay Kits are available with wells precoated with Collagen I, Fibronectin or Laminin to promote adhesion of certain cell types.
Indigo Biosciences Human Adrenoceptor Alpha 1B (ADRA1B) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 384-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1B reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1B. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Enzo Life Sciences Blebbistatin(+/-), (5mg), CAS Number: 674289-55-5
Inhibitor of non-muscle myosin II. Blebbistatin (so named because of its ability to block cell blebbing) is a selective and high-affinity (IC50 approx. 4 µM) inhibitor of non-muscle myosin II. During cell division blebbistatin inhibits contraction of the cleavage furrow without disrupting mitosis. It inhibits pancreatic adenocarcinoma cellular invasiveness and PDGF-stimulated fibroblast-collagen matrix contraction. Formula: C18H16N2O2. MW: 292.3. CAS Number: 674289-55-5. Purity: ≥97%. Appearance: Bright yellow solid. Solubility: Soluble in DMSO (25mg/ml). Long Term Storage: -20°C
Eurofins Discoverx PathHunter eXpress PDGFRb Functional Assay, 10-Plate Kit. (93-0493E3CP16L)
The PathHunter eXpress PDGFRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PDGFRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Medchemexpress LLC PLAU/uPA Rat HEK29 10ug
PLAU/uPA proteins lack conserved residues critical for feature annotation propagation suggesting potential differences in molecular interactions in plasminogen activation and fibrinolysis regulation PLAU/uPA Protein Rat (HEK293 His) is the recombinant rat-derived PLAU/uPA protein expressed by HEK293 with C-His labeled tag
Cayman Chemical ALT asy InItIatr 1ea
A - and K1-opioid receptor antagonist (Kis 0.3 and 0.2 nM respectively) selective for - and K1-opioid receptors over the -opioid receptor (Ki 12.8 nM) reversibly inhibits electricity-induced twitches of isolated guinea pig ileal longitudinal muscle strips (IC50 48 nM) indicating opioid agonist activity inhibits endomorphin 1-induced conditioned place preference in mice at 0.3 UG/animal i.c.v. prevents decreases in urine output induced by fentanyl D-propoxyphene buprenorphine profadol or bromadoline in water-loaded rats at 40 mg/kg reduces hemokinin 1-induced increases in the latency to withdrawal in the tail-flick test in mice
ABclonal Technology β-Actin Rabbit pAb
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Eurofins Discoverx PathHunter eXpress P2RY11 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0281E2CP2L)
The PathHunter P2RY11 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ATP) based activation of P2RY11 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY11 and EA-tagged β-Arrestin-2. Activation of P2RY11 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.