General Cell Based Assays
Eurofins Discoverx PathHunter eXpress IL17RD/TNFR2 Dimerization Assay, 2pl Kit. (93-1068E3CP5M)
The PathHunter IL17RD/TNFR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TNFα) induced dimerization of IL17RD/TNFR2 receptors. This product uses EFC technology. The cell line included overexpresses IL17RD target protein fused to PK alongside TNFR2 target protein fused to EA. Upon ligand-induced dimerization of IL17RD/TNFR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 CHO-K1 β-Arrestin-1 GPCR Assay, 2pl Kit. (93-1050E2CP2M)
The PathHunter AGTRL1 (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-1. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OSMR/IL6ST Dimerization Assay, 10pl Kit. (93-1056E3CP5L)
The PathHunter OSMR/IL6ST Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. oncostatin M) induced dimerization of OSMR/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside OSMR target protein fused to EA. Upon ligand-induced dimerization of OSMR/IL6ST, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVR1C/ACVR2B Dimerization Assay, 10pl Kit. (93-1047E3CP5L)
The PathHunter ACVR1C/ACVR2B Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. activin B) induced dimerization of ACVR1C/ACVR2B receptors. This product uses EFC technology. The cell line included overexpresses ACVR2B target protein fused to PK alongside ACVR1C target protein fused to EA. Upon ligand-induced dimerization of ACVR1C/ACVR2B, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL18R1/IL18RAP Dimerization Assay, 10pl Kit. (93-1044E3CP5L)
The PathHunter IL18R1/IL18RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-18) induced dimerization of IL18R1/IL18RAP receptors. This product uses EFC technology. The cell line included overexpresses IL18R1 target protein fused to PK alongside IL18RAP target protein fused to EA. Upon ligand-induced dimerization of IL18R1/IL18RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 CHO-K1 β-Arrestin-1 GPCR Assay, 10pl Kit. (93-1050E2CP2L)
The PathHunter AGTRL1 (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-1. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1F U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0699E3CP0M)
The PathHunter HTR1F eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM3 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0860E3CP0L)
The PathHunter CHRM3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM3 and EA-tagged β-Arrestin-2. Activation of CHRM3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 Activated GPCR Internalization Assay, 10pl Kit. (93-0710E3CP0L)
The PathHunter AGTRL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of AGTRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1F U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0699E3CP0L)
The PathHunter HTR1F eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BLK Activity Assay, 2-Plate Kit. (93-0882E3CP16M)
The PathHunter eXpress BLK Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the BLK kinase domain. The cell line is engineered to express the catalytic domain from BLK fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the BLK catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TORC1 Nuclear Translocation Assay, 10pl Kit. (93-0833E3CP19L)
The PathHunter eXpress TORC1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC1 (CRTC1) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC1 (CRTC1) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL1RL1/IL1RAP Dimerization Assay, 2pl Kit. (93-1067E3CP5M)
The PathHunter IL1RL1/IL1RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-33) induced dimerization of IL1RL1/IL1RAP receptors. This product uses EFC technology. The cell line included overexpresses IL1RL1 target protein fused to PK alongside IL1RAP target protein fused to EA. Upon ligand-induced dimerization of IL1RL1/IL1RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.