General Cell Based Assays
Agilent Technologies SEAHORSE XF T CELL META KIT
Seahorse XF T Cell Metabolic Profiling KitSeahorse XF T Cell Metabolic Profiling Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of oligomycin A, BAM15, and rotenone/antimycin A. For use with Seahorse XF Pro and XFe96 analyzers.Assay Wells 6 x 96-wellAssays per Kit: 6Shipping Condition: Ambient TemperatureStorage Temp Conditions: Room temperature
Cayman Chemical Glycerol Colorimetric Assay K
Glycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in various conditions. The measurement of glycerol is also useful for correction of glycerol interference when measuring triglycerides in reference materials and in serum from patients with elevated glycerol concentrations. Cayman’s Glycerol Assay provides a simple, reproducible, and sensitive tool for measurement of glycerol in plasma and serum. The assay employs a coupled enzymatic reaction system that yields a brilliant purple product with an absorbance maximum at 540 nm.
Eurofins Discoverx cAMP Hunter eXpress GPR109A CHO-K1 GPCR Assay, 2-Plate Kit. (95-0122E2CP2M)
The cAMP Hunter GPR109A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR109A, a naturally Gi/Go coupled GPCR. Upon activation of GPR109A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCXCR4 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0481E3MCP5L)
The PathHunter mCXCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR4 and EA-tagged β-Arrestin-2. Activation of mCXCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 3 Reagent, 500. (93-0563R3B)
AssayComplete Cell Plating 3 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Biochain Institute Inc NAD/NADH Assay Kit, 100 Assays/PK
BioChain's NAD+/NADH assay kit provides simple, direct and automation-ready procedure to measure NAD+/NADH concentration. It based on an alcohol dehydrogenase cycling reaction, in which a tetrazolium dye (MTT) is reduced by NADH in the presence of phenazine methosulfate (PMS). The intensity of the product color, measured at 565 nm, is proportionate to the NAD+/NADH concentration in the sample.
AdipoGen Insulin human ELISA Kit
Colorimetric Sandwich ELISA Assay. Detects human Insulin in serum, plasma and cell culture supernatant. Range 7.81 to 500pg/ml. Sensitivity 10 pg/ml. Samples Cell Culture Supernatant, Plasma, Serum. Glucose is the main fuel for the human body and blood glucose values are tightly regulated in healthy individuals. Regulation of glucose uptake in cells is performed by the hormone called insulin which is glucose-responsively secreted from pancreatic beta cells. When the glucose concentration in the blood is increased, insulin lowers it by increasing glucose uptake by muscle, liver and fat cells. Insulin is synthesized by beta cells of the pancreas in the form of a single chain of three peptides B, C and A in the order B-chain-C peptide-A-chain. The mature insulin consists of B-chain 30 amino acids and A-chain 21 amino acids linked by two inter-chain and one intra-chain disulfide bridge.
Meso Scale Discovery U-PLEX Mouse IL-5 ASY
The Meso Scale Discovery U-PLEX Mouse IL-5 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 5 (IL-5) in serum plasma and cell culture supernatants It uses MSD s SECTOR Imager detecting IL-5 from 0 04 to 1000 pg/mL The kit includes a 96-well plate with capture antibody detection antibody with MSD SULFO-TAG calibrators and diluent The assay takes 3 5 hours and supports research on mouse models of asthma allergies and other IL-5-related immunological diseases
Meso Scale Discovery U-PLEX Human ICAM-1 ASY
The Meso Scale Discovery U-PLEX Human ICAM-1 Assay Kit is designed to quantitatively measure Intercellular Adhesion Molecule 1 (ICAM-1) in human serum plasma and cell culture supernatants using an electrochemiluminescent detection method This assay kit includes a 96-well plate coated with capture antibody detection antibody conjugated to an electrochemiluminescent label calibrator and buffer for sample dilution The assay has a detection range of 78-50 000 pg/mL and exhibits no cross-reactivity with other adhesion molecules This product can aid in understanding ICAM-1 levels related to inflammatory and immune responses
Meso Scale Discovery U-PLEX Mouse VEGF-a ASY
The Meso Scale Discovery U-PLEX Mouse VEGF-A Assay Kit is a multiplex immunoassay for measuring mouse VEGF-A in various biological samples It uses electrochemiluminescence (ECL) detection for high sensitivity and precision The kit includes a 96-well plate with capture antibodies ECL-tagged detection antibodies calibrators and buffers It quantifies mouse VEGF-A concentrations from 0 53-2000 pg/mL with minimal sample volume aiding in biomarker research
Eurofins Discoverx PathHunter eXpress CCR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0651E3CP5L)
The PathHunter CCR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.