General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GPR20 HEK 293 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0405E1CP1M)
The PathHunter GPR20 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR20 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR20 and EA-tagged β-Arrestin-2. Activation of GPR20 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mGHSR1a CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0605E2MCP2L)
The PathHunter mGHSR1a eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. grehlin) based activation of mGHSR1a GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGHSR1a and EA-tagged β-Arrestin-2. Activation of mGHSR1a stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 16 Reagent, 100. (93-0563R16A)
AssayComplete Cell Plating 16 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress NTSR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0280E2CP0M)
The PathHunter NTSR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neurotensin) based activation of NTSR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NTSR1 and EA-tagged β-Arrestin-2. Activation of NTSR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL4R/IL13RA1 Dimerization Assay, U2OS, 2pl Kit. (93-1000E3CP5M)
The PathHunter IL4R/IL13RA1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-13) induced dimerization of IL4R/IL13RA1 receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL13RA1 target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL13RA1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human Total SMAD1 Detection Kit, 50,000 Assay Points
HTRF Human Total SMAD1 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser239) Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-VASP (Ser239) Detection Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress YES1 Activity Assay, 2-Plate Kit. (93-0883E3CP16M)
The PathHunter eXpress YES Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the YES kinase domain. The cell line is engineered to express the catalytic domain from YES fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the YES catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter TrkC Bioassay Kit, 10-Plate. (93-0464Y3-00078)
The PathHunter TrkC Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. NT-3) based activation of TrkC receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged TrkC target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Sigma Aldrich Fine Chemicals Biosciences Direct DetectR Assay free
Quantity OptionsDDAC00010-GR 50 CardsDDAC00010-4P 200 CardsDDAC00010-8P 400 CardsThe Direct Detect Assay-free Cards are desgined for use in the the Direct Detect Spectrometer. The card contains four hydrophilic polytetrafluoroethylene (PTfE) membrane positions sized for easy sample application and surrounded by a hydrophobic ring to retain analyzed sample within the IR beam. A 2 muL sample volume is recommended in order to assure proper sample application drying and measurement.The Assay-free card can be inserted into the system and analyzed immediately after preparation. The Direct Detect spectrometer has a built in drying system that will automatically dry the sample to the point where it will give an accurate reading. The Direct Detect Assay-free card is intended for a one-time protein application however already prepared cards can be stored and reread later.
Eurofins Discoverx cAMP Hunter eXpress MC3R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0045E2CP2L)
The cAMP Hunter MC3R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-melanocyte stimulating hormone) based activation of MC3R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MC3R, a naturally Gs coupled GPCR. Upon activation of MC3R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR5 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0402E3CP0M)
The PathHunter SSTR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR5 and EA-tagged β-Arrestin-2. Activation of SSTR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Hach Company SC4500 Ctrl Prog Prof DP 1 mA
SC4500 Controller-Prognosys-Profibus DP-1 mA Input-100-240 VAC-without plug C1D2Includes Controller-Non-Claros Embedded Prognosys-without power cord includes mounting hardware
Meso Scale Discovery U-PLEX Mouse IL-4 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-4 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 4 (IL-4) in various samples It uses MSD s SECTOR Imager to detect signals from microplate wells The kit includes a capture antibody for IL-4 and a ruthenium-labeled detection antibody It has a detection range of 0 05-1000 pg/mL with no cross-reactivity with other cytokines The kit provides a 96-well plate detection antibody calibrators 10X diluent and components for 96 tests
Meso Scale Discovery RPLEX HUM Cytokeratin19 ASY
The R-PLEX Human Cytokeratin 19 Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that quantitatively measures cytokeratin 19 levels in human serum plasma and cell culture supernatant samples The assay utilizes electrochemiluminescent detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibodies detection antibodies calibrators and reagents required for the assay It can be used for cancer biomarker research and measuring treatment response The assay has a lower limit of detection of 0 016 ng/mL and an upper limit of quantification of 400 ng/mL for cytokeratin 19