General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress GPR35 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0152E2CP2M)
The cAMP Hunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR35, a naturally Gi/Go coupled GPCR. Upon activation of GPR35, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress mGLP1R U2OS GPCR Assay, 10-Plate Kit. (95-0179E3CP5L)
The cAMP Hunter mGLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type mGLP1R, a naturally Gs coupled GPCR. Upon activation of mGLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Cysteinyl Leukotriene Express
Cayman’s CysLT Express ELISA Kit is a competitive assay that can be used for quantification of CysLTs in urine, plasma, serum, lavage fluids and aspirates, culture media, and other sample matrices. The assay has a range of 7.8-1,000 pg/ml, an average sensitivity (80% B/B0) of 24 pg/ml, and a lower limit of detection (LLOD) of 6.4 pg/ml. NOTE: This kit measures all CysLTs. For measurement of specific CysLTs, please see Cayman Chemical’s Leukotriene E4 ELISA Kit (Item No. 501060) and Leukotriene C4 ELISA Kit (Item No. 501070).
AMERICAN RESEARCH PRODUCTS INC ALKALINE PHOSPHATASE ASSAY KIT
503029981 ALKALINE PHOSPHATASE ASSAY KIT
AMERICAN RESEARCH PRODUCTS INC REACTIVE OXYGEN SPECIES ASSAY
503029925 REACTIVE OXYGEN SPECIES ASSAY
Meso Scale Discovery U-PLEX Mouse MIP-1 ASY
The Meso Scale Discovery U-PLEX Mouse MIP-1alpha Assay Kit is a multiplex immunoassay that measures mouse macrophage inflammatory protein 1-alpha (MIP-1alpha) in serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and diluent The assay takes approximately 3 5 hours to run and 10 L of sample is required The kit can test up to 80 samples in duplicate
Cayman Chemical Cel-Based asy DIgItonIn So 5ea
An OGA inhibitor (IC50 46 nM) selective for OGA overBhexosaminidase (IC50 >10 UM) and a panel of 277 kinases (IC50s >1 UM for all) increases the levels of O-GlcNAcylated proteins in SH-SY5Y cells (EC50 450 nM)
Cell Biolabs Inc Total Cholesterol Assay Kit (Fluorometric), 192 assays
Cell Biolabs’ Total Cholesterol Assay Kit is a simple fluorometric assay that measures the amount of total cholesterol present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, cholesterol standards and unknown samples. Sample cholesterol concentrations are determined by comparison with a known cholesterol standard. Cholesteryl esters can be quantified by subtracting the free cholesterol values from the total cholesterol value.
Meso Scale Discovery U-PLEX Human MCP-1 ASY 25 PL
The Meso Scale Discovery U-PLEX Human MCP-1 Assay Kit quantifies monocyte chemoattractant protein 1 (MCP-1) in human serum plasma and cell culture supernatants Using MSD s SULFO-TAG labels and multi-array technology it measures MCP-1 levels down to 0 4 pg/mL The kit includes a 96-well plate with capture antibodies detection antibodies buffers blockers calibrators and controls The assay takes about 3 5 hours and requires an MSD instrument like the MESO QuickPlex SQ 120 It offers high sensitivity broad range and minimal sample volume for efficient MCP-1 analysis