General Cell Based Assays
Enzo Life Sciences FLUOR DE LYS SIRT5 fluorometric drug discovery assay kit (100 tests)
The SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats, including cuvettes and 384-well plates. The SIRT5 Fluorometric Activity Assay is based on the unique FLUOR DE LYS-Succinyl Substrate/Developer combination. The FLUOR DE LYS-Succinyl Substrate, which comprises a single lysine residue, Nε-succinylated on its side-chain, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS-Succinyl sensitizes it so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Sirtuin 5 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-SMAD2 (Ser465/467 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-SMAD2 (Ser465/467 & Total) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc ViaStain Live Caspase 3/7 Detection for 2D/3D Culture with Hoechst
ViaStain Live Caspase 3/7 Detection for 2D/3D Culture with Hoechst
Cayman Chemical SIRT1 FRETBased Screening Ass
Human SIRT1 is the homolog of yeast Sir2 and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Cayman’s SIRT1 FRET-based Screening Assay provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two EAsy steps, both performed in the same microplate. In the first step, the substrate, which is coupled to the fluorophore and quencher, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step results in the separation of the quencher and fluorophore. The resulting fluorescence is analyzed using an excitation wavelength between 335-345 nm and emission wavelength between 440-465 nm.
Eurofins Discoverx PathHunter eXpress TrkA Functional Assay, 10-Plate Kit. (93-0462E3CP16L)
The PathHunter eXpress TrkA Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkA dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkA and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR171 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0415E2ACP1M)
The PathHunter GPR171 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR171 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR171 and EA-tagged β-Arrestin-2. Activation of GPR171 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay, 10-plate Kit. (96-0046E1CP7L)
The InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRDT(1,2) via detection of BRDT(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRDT(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter SSTR2 Bioassay Kit, 2-Plate. (93-0181Y2-00067)
The PathHunter SSTR2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. somatostatin 28) based activation of SSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR2 and EA-tagged β-Arrestin-2. Activation of SSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery U-PLEX Human IL-17D ASY
The Meso Scale Discovery U-PLEX Human IL-17D Assay Kit is a multiplex immunoassay for measuring IL-17D in human serum plasma and cell culture supernatant It uses electrochemiluminescence detection offering high sensitivity a wide dynamic range and low sample volume needs The kit includes a 96-well plate with capture antibodies detection antibodies with electrochemiluminescent labels calibrators and diluents It quantifies IL-17D with minimal hands-on time using an MSD instrument providing a simple rapid method to measure IL-17D important for immune regulation
Revvity Health Sciences Inc HTRF Human & Mouse PARP cleaved-Asp214 Detection Kit, 500 Assay Points
HTRF Human & Mouse PARP cleaved-Asp214 Detection Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress GPR31 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0406E2CP2M)
The PathHunter GPR31 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR31 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR31 and EA-tagged β-Arrestin-2. Activation of GPR31 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR141 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0385E2ACP1L)
The PathHunter GPR141 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR141 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR141 and EA-tagged β-Arrestin-2. Activation of GPR141 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mUTR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0525E2MCP0M)
The PathHunter mUTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. urotensin II) based activation of mUTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mUTR2 and EA-tagged β-Arrestin-2. Activation of mUTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDG7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0636E2CP13L)
The PathHunter EDG7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. LPA) based activation of EDG7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG7 and EA-tagged β-Arrestin-2. Activation of EDG7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.