RFLG‑45A is an affinity‑purified rabbit polyclonal antibody targeting the DYKDDDDK (FLAG) epitope, widely used in recombinant protein expression and detection. The purification process yields high‑specificity antibodies with minimal background, supporting clear and reproducible results in Western blotting, ELISA, immunoprecipitation, and immunofluorescence. This reagent provides strong, selective binding to FLAG‑tagged proteins, ensuring dependable performance across diverse assay conditions. Manufactured by Immunology Consultants and supplied in a 1 mg format, RFLG‑45A is an essential tool for researchers working with FLAG‑tagged constructs.