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The HI100 monoclonal antibody binds to the 220 kDa isoform of the human leukocyte common antigen. CD45RA is an isoform of CD45 due to alternate splicing1. CD45RA is expressed on naïve or resting CD4+ and CD8+ T cells, as well as on a subset of activated T cells2,3. The associated tyrosine phosphatase activity regulates signal transduction processes during hematopoiesis and immune responses4.The antibody was conjugated to a fluorophore and purified by affinity chromatography.
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XK empty columns provide reliable and reproducible separations. The tube material is of borosilicate glass which allows for visual inspection of the media bed. The columns are made of biocompatible materials that exhibit excellent chemical resistance. A broad range of column dimensions makes XK columns suitable for many different purification needs. Column packing can be performed using either a packing reservoir or an extra column tube attached with a packing connector. The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter simplifying adjustments of the bed height disassembly and cleaning. The design of the adapter plunger gives a uniform flow which maintains the integrity of the packed bed during operations. An extensive range of spare parts is available facilitating maintenance.
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AAVpro Purification Kit Midi (All Serotypes),4 Preps.The AAVpro Purification Kit Midi (All Serotypes) is a four-prep kit that can be used for purifying AAV of any serotype without the need for ultracentrifugation. The purification yields highly pure AAV particles that can be used directly for in vivo transduction or transduction of cultured cells. Cryonase Cold-Active Nuclease is included. This product allows for purification of four virus preps, each from a culture size of 1 x T225 flask.
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Because polystyrene transfers sonic wave energy much more efficiently than polypropylene, Active Motif highly recommends that its Polystyrene Sonication Tubes be used with its Q800R Sonicator. Use of polypropylene tubes in indirect sonication has been shown to reduce the extent to which the chromatin is sheared
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This recombinant monoclonal mouse anti-6X His tag with CF and reg 568 dye CF and reg dyes are Biotiums line of next-generation fluorescent dyes with advantages in brightness photostability and conjugate specificity CF and reg 568 dye has excitation/emission at 562/584 nm
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