Protein Purification
STEMCELL Technologies EasySep Buffer, 1 L
Cell separation buffer for in vitro diagnostic IVD applications
AdipoGen IDO1 mouse rec His
Protein. Mouse IDO aa 1-407 is fused at the C-terminus to a His-tag. Source E. coli. Endotoxin content <1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in 50mM TRIS-Cl, pH 7.4, containing 1mM EDTA. Purity >90% SDS-PAGE. IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic NOD mice, and as a protective negative regulator in autoimmune disorders.
AdipoGen NAPRTase human rec His
Protein. Human NAPRTase is fused at the N-terminus to a His-tag. Source E. coli. Endotoxin content N/A. Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT. Purity >98% SDS-PAGE. Human nicotinate phosphoribosyltransferase NAPRTase is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid NA to NA mononucleotide NaMN and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD biosynthesis pathway. Catalytic activity Beta-nicotinate D-ribonucleotide diphosphate = nicotinate 5-phospho-alpha-D-ribose 1-diphosphate.
Sigma Aldrich Fine Chemicals Biosciences Lipopolysaccharides from Escherichia coli O111:B4 purified by ion-exchange chromatography, TLR ligand tested, MFCD00164401
Lipopolysaccharides from Escherichia coli O111:B4 purified by ion-exchange chromatography, TLR ligand tested
Sigma Aldrich Fine Chemicals Biosciences Lysis solution for blood | 2.5ML
Lysis solution for blood | 2.5ML
Sigma Aldrich Fine Chemicals Biosciences Triton X-100 laboratory grade, 9002-93-1, MFCD00128254, 500mL
Linear Formula t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10, laboratory grade, Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Abcam Cyclic pifithrin-alpha-p-nitro, transcriptional p53 inhibitor, 5MG
MW 299.3 Da, Purity >99%. Cell-permeable transcriptional p53 inhibitor. Shows potent neuroprotective effects (ED₅₀ = 30 nM, etoposide-induced cortical neuron death). Shows a 50% longer half-life than Pifithrin-α (ab120478).
The product is subject to the following: Abcam Restricted Use Statement
Abcam SAHA (Vorinostat), HDAC inhibitor, 50MG
MW 264.32 Da, Purity >98%. Potent non-selective HDAC inhibitor (IC₅₀ values are 10 and 20 nM for HDAC1 and HDAC3, respectively). Induces apoptosis and shows antiproliferative effects against cancer cell lines (IC₅₀ = 3 - 8 μM). Antitumor effects in vivo. No effect on class III HDACs. Orally active.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Suberoyl bis-hydro x amic acid (SBHA), HDAC inhibitor, 500MG
MW 204.22 Da, Purity >98%. Potent, non-selective HDAC inhibitor (ID₅₀ values are 0.25 and 0.3 μM for HDAC1 and HDAC3 respectively). Potentiates the cytostatic effects of 5-Fluorouracil (ab142387). Induces apoptosis. Shows antiproliferative effects (IC₅₀ = 15 μM, HeLa cells).
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies EasySep™ HLA Chimerism BC CD56 Positive Selection Kit, For processing: 30 mL buffy coat processed
The EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells and NKT cells.This product replaces the EasySep™ Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA), for even faster cell isolations.
STEMCELL Technologies EasySep™ Direct Human CD4+ T Cell Isolation Kit for RoboSep™, For processing: 100 mL whole blood processed
Isolate CD4+ T cells directly from human whole blood by immunomagnetic negative selection. Desired cells undergo minimal manipulation and are untouched and highly purified.This product labels RBCs, platelets and unwanted cells with antibody complexes and magnetic particles. The magnetically labeled RBCs, platelets and non-target cells are separated from the untouched CD4+ T cells in an EasySep™ magnet and poured or pipetted into a new tube.Isolated cells are available for use in downstream uses such as flow cytometry, functional assays or culture.
STEMCELL Technologies RoboSep™ Mouse CD90.2 Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD90.2 Positive Selection Kit II is designed to isolate CD90.2+ (Thy1.2+) cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD90.2 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD90.2 Positive Selection Kit (Catalog #18751) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
Abcam RKRARKE, PKG Inhibitor, 1MG
MW 943.12 g/mol, Purity >99%. Competitive protein kinase G (PKG) inhibitor. Ki = 86 µM for PKG; Ki = 550 µM for PKA. Potential inhibitory activity in neuronal systems.
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. NEBExpress Ni Resin – 25 ml
NEBExpress Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and beta-Mercaptoethanol.
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit is designed to isolate highly purified CD15+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD15 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD15 antigen is expressed on granulocytes and monocytes.This product replaces the EasySep™ Human Whole Blood CD15 Positive Selection Kit (Catalog #18681) and EasySep™ HLA Whole Blood CD15 Positive Selection Kit (Catalog #18681HLA), for even faster cell isolations.