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Miscellaneous Immobilized Affinity Ligands

Agarose beads and other resins bound to specialized immobilized ligands for use in diverse affinity procedures; ligands may include biotin, poly(e-lysine), lectins, metal ions, ribonucleotides, heparin, etc.; suitable for target molecule purification.
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1

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Shipping Condition Wet Ice
Assay End-point Chromogenic Assay
Type Endotoxin Quantitation Kit
Target Endotoxins
Content And Storage • Lyophilized amebocyte lysate
• E.coli endotoxin standard
• Chromogenic substrate
• Endotoxin-free water

Store at 2–8°C upon receipt.
Format Kit
Assay Sensitivity 0.01 EU/mL
Assay Range 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line Pierce
Detection Method Colorimetric
2

Thermo Scientific™ Pierce™ p-Aminophenyl Phosphoryl Choline Agarose

Bind and purify C-reactive protein (CRP) using these agarose beads of immobilized p-aminophenyl phosphoryl choline (APC).

EDGE
3

Novus Biologicals™ LPS from E. Coli, TLR4 ligand
SDP

Applications: Functional

4

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.25 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

5

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Stationary Phase Proprietary Ligand
Type Spin Column
Target Endotoxin Removal
Content And Storage Upon receipt store at 4°C.
Format Spin Column
Form Liquid
Column Type Affinity, Cellulose Resin
Formulation 22mL-capacity centrifuge columns containing 1mL resin
Capacity (Metric) Up to 2,000,000 EU/mL
Product Line Pierce
6

Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

Stationary Phase Polymixin B
Type Endotoxin Removing Gel
Target Endotoxin Removal
Content And Storage Store at 4°C.
Format Bottle
Column Type Affinity, Agarose Resin
Capacity (Metric) 4000 to 6000 EU Binding per mL Gel
Product Line Detoxi-Gel
7

Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Columns, 1 mL

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

8

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin
GSA_VA

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Stationary Phase Proprietary Ligand
Type Resin
Target Endotoxin Removal
Content And Storage Upon receipt store at 4°C.
Format Bottle
Column Type Affinity, Proprietary Resin
Formulation Proprietary ligand on beaded cellulose slurried in 20% ethanol
Capacity (Metric) Up to 2,000,000 EU/mL
Product Line Pierce
9

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.5 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Stationary Phase Proprietary Ligand
Type Spin Column
Target Endotoxin Removal
Content And Storage Upon receipt store at 4°C.
Format Spin Column
Form Liquid
Column Type Affinity, Cellulose Resin
Formulation 8mL-capacity centrifuge columns containing 0.5mL resin
Capacity (Metric) Up to 2,000,000 EU/mL
Product Line Pierce
10

Invitrogen™ EK-Away™ Resin

Specifically designed for removal of EKMaxor other enterokinase enzymes following cleavage of proteins containing the enterokinase cleavage site

ENCOMPASS_PREFERRED
Stationary Phase Enterokinase
Type Resin
Content And Storage EK-Away™ is provided as a 50% ethanol slurry and should be stored at +4°C. 10X binding and 10X stripping buffers are included. 7.5 ml resin will remove 250 units of enterokinase; 30 ml will remove 1000 units of EKMax™. All reagents are guaranteed stable for 6 months when stored properly.
Form Liquid Suspension
Column Type Affinity
Product Line EK-Away
11

Thermo Scientific™ Pierce™ Iminobiotin Agarose

Agarose beads of immobilized biotin (and iminobiotin) to strongly (or reversibly) bind and remove (or purify) streptavidin or avidin proteins and conjugates from samples.

12

Novus Biologicals™ Pam3CSK4, TLR1 and TLR2 Ligand
SDP

Applications: Functional, In vitro assay, In vivo assay, Ligand Activation

Target Pam3CSK4, TLR1 and TLR2 Ligand
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Concentration 0.5 mg/mL
For Use With (Application) Activation of TLR1 and TLR2 heterodimer. Stimulation of TLR1 and TLR2 has been reported with 10 to 100ng/mL. Use in vitro assay reported in scientific literature (PMID: 24105263) Use in functional reported in scientific literature (PMID 25511699). Use in In-vivo reported in scientific literature (PMID 25466255). Use in ligand activation reported in multiple pieces scientific literature.
Species Human/Mouse
13

Novus Biologicals™ Zymosan, TLR2 Ligand
SDP

Applications: Functional, In vitro assay, In vivo assay, Ligand Activation

14

Thermo Scientific™ Pierce™ D-Galactose Agarose

Bind and affinity-purify lectins, galactosidases and other galactose-binding molecules using this beaded agarose resin with immobilized thio-alpha-D-galactose.

15

Thermo Scientific™ Pierce™ Boronic Acid Resin

Selectively bind and remove or purify ribonucleotides and small oligonucleotide RNAs using polyacrylamide beads of immobilized m-aminophenyl-boronic acid.