Protein Purification Reagents and Kits
Genscript Corporation High Affinity Ni-Charged Resin
GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2 by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins
INTACT GENOMICS INC PE pegRNA Synthesis Kit 10 Reactions (100 µl RXN Volume)
The IG PE pegRNA Synthesis Kit is a perfect choice for a variety of pegRNA synthesis needs. Below is a sampling of some of the key benefits. IG PE pegRNA Synthesis Kit includes everything but your target oligos (all buffers, enzymes, scaffolds). User simply needs to provide the oligos for your target: a pair of sgRNA oligo and pegRTpbs oligo generated using the template design in this manual. Rapid Workflow (Less than 1 hour) Scalable Yield (100 µl reaction)
Genscript Corporation AmMag™ Wand D12
GenScript’s AmMag Wand enables protein and antibody purification from cultures up to 2 L in volume using the AmMag SA. Magnetic beads are directly incubated in cultures or lysates to bind target proteins and antibodies. Once binding is complete, the AmMag Wand is used to collect the beads and transfer them into 50 ml tubes, to be washed and eluted automatically using the AmMag SA purification system, or manually using the AmMag MR magnetic racks.
Sigma Aldrich Fine Chemicals Biosciences DEAE-Sepharose(R) Fast Flow | 57407-08-6 | MFCD00146630 | 50ML
DEAE-Sepharose(R) Fast Flow | 57407-08-6 | MFCD00146630 | 50ML
STEMCELL Technologies RoboSep™ Human Pan-DC Pre-Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human Pan-DC Pre-Enrichment Kit is designed to pre-enrich all dendritic cells (DCs) (including myeloid and plasmacytoid DCs) from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-pan DCs and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Abcam Mitochondria Isolation Kit For Tissue and Cultured Cells
Mitochondria Isolation Kit For Tissue and Cultured Cells
The product is subject to the following: Abcam Restricted Use Statement
Biolegend MojoSortTM Mouse F4/80 Selec
MojoSort™ Mouse F4/80 Selection Kit Reactivity: Mouse; Apps: Cell Sep - Pos; Size: 10 tests
Zymo Research Corporation Zymo-Spin™ ChIP Kit (25 preps) Mechanical Shearing Protocol '*'
This ChIP protocol involves covalent cross-linking of protein-DNA complexes with formaldehyde followed by cell lysis and chromatin shearing. A ChIP-grade antibody (user supplied) is used with Protein A magnetic beads to immunoprecipitate the protein-DNA complexes of interest. Following reverse crosslinking, RNase A and Proteinase K treatments, the DNA is eluted in a minimal volume of buffer using a unique micro-elution spin column, eliminating the need for messy precipitations. The protocol has been optimized for efficient crosslinking, shearing, and immunoprecipitation regardless of the mammalian cell type. Additionally, eluted ChIP DNA is ideal for end point PCR, quantitative PCR, ChIPSeq library preparation, and Next-Gen sequencing-based applications.
Abcam Lysosome Isolation Kit
Lysosome Isolation Kit
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences CM Sepharose | 68894-07-5 | MFCD00146478 | 50 mL
CM Sepharose | 68894-07-5 | MFCD00146478 | 50 mL
Genscript Corporation Glutathione Resin
Glutathione Resin (Cat. No. L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications. Table 1 lists the main characteristics of Glutathione Resin.
STEMCELL Technologies EasySep™ Human Resting CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Resting CD4+ T Cell Isolation Kit is designed to isolate resting CD4+ T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit, For processing: 500 mL cord blood processed
Includes RosetteSep™ human cord blood progenitor kit and HetaSep™
STEMCELL Technologies EasySep™ Mouse Biotin Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556). For highly purified particle-free cells, we recommend the EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655). (https://www.stemcell.com/products/easysep-release-mouse-biotin-positive-selection-kit.html)
Agilent Technologies AdvanceBio total sialic acid quantitation kit, 48/pk
Rapid quantitation of total sialic acid by fluorescence or absorbance detection, either free or released from intact glycoproteins. Automatable, 96-well microplate format with fluorescence detection. Broad range of detection, 40 pmol - 1,000 pmol. Sample digestion, conversion, detection and quantitation may be performed in a single well for fast and simple processing. Enzymatic cleavage allows rapid analysis (~75 minutes or less) with minimal, degradation of sialic acid.