Protein Purification Reagents and Kits
Aat Bioquest Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
STEMCELL Technologies RoboSep™ Human Neutrophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Neutrophil Isolation Kit isolates functional, highly purified neutrophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.This product replaces the EasySep™ Human Neutrophil Isolation Kit (Catalog #19257) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse TIL (CD45) Positive Selection Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse TIL (CD45) Positive Selection Kit is designed to isolate highly purified CD45+ tumor infiltrating leukocytes (TILs) by immunomagnetic positive selection. This kit has been optimized for use on single-cell suspensions of solid mouse tumors with a low CD45+ start cell frequency, including tumors induced by implantation of 4T1, B16-F10, and CT26.WT cell lines into syngeneic mice. Due to the heterogeneity of mouse tumors, this kit may require optimization.This kit targets CD45+ cells for positive selection with antibodies recognizing the CD45 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, and cell-based experiments.
Perkin Elmer US LLC Low Flow Cold Trap Tube for ATD 400
Includes the following pieces: 1 Low Flow Trap Tube (L4271298) 6 Trap Packing Discs (L4271290)
Active Motif ChIP-IT Express
ChIP-IT Kits provide all of the critical components needed for successful ChIP in a single kit. ChIP-IT comes complete with positive & negative control antibodies and PCR primers, DNA purification columns, optimized buffers and a comprehensive manual, all of which have been proven to work in ChIP. For your convenience, kits are available in a variety of formats, with your choice of sonication or our exclusive enzymatic shearing, and either with or without positive & negative controls. This enables you to use the shearing method that works best for you, and to either receive human-specific controls with the kit or to purchase ChIP-IT Control Kits separately to help analyze your results and verify that your antibodies work in the species you choose.
Abcam ChIP Kit - One Step, 96 Tests
Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA.
The product is subject to the following: Abcam Restricted Use Statement
IBA LifeSciences Strep-TactinXT 4Flow resin; 50 ml
Strep-TactinXT 4Flow is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow enables the application of denaturing agents such as urea. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
Thermo Scientific™ RIPA Extraction Bundle
RIPA Extraction Bundle effectively lyses and extracts proteins from cultured mammalian cells and provides high-precision total protein concentration determination. The bundle includes RIPA Lysis and Extraction Buffer, Halt Protease Inhibitor Cocktail and Pierce Rapid Gold BCA Protein Assay kit
Cytiva Mono Q PC, Columns Prepacked with MonoBeads™
Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. Cytiva Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles.
Thermo Scientific™ SureQuant™ RAS Isoform Relative Quantitation Module
Thermo Scientific™ SureQuant™ RAS Isoform Relative Quantitation Module provides a complete quantitation solution for the analysis of key RAS expression using mass spectrometry (MS).
Thermo Scientific™ NeuCode™ Lysine-602
Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.
Cytiva StrepTrap™ HP Prepacked Columns
Purify Strep(II)-tagged recombinant proteins by affinity chromatography. Cytiva Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.
MilliporeSigma™ Calbiochem™ LCAT Activity Assay Kit (Fluorometric)
Useful for quantitative measurement of lecithin, cholesterol acyltransferase activity.