Protein Purification Reagents and Kits
IBA LifeSciences Strep-TactinXT APC , 50 µl
Strep-TactinXT conjugated to allophycocyanin (APC) is optimized for flow cytometry of cells. It binds to Strep-tagII- or Twin-Strep-tag- fusion proteins with a high affinity.Cells can either be stained directly if they express a strep-tagged surface protein or indirectly via a strep-tagged protein that subsequently binds to the cells (e.g. for sorting antigen-specific B cells).Independent from the chosen method, biotin addition will cause the release of Strep-TactinXT APC from the cell surface. This enables the use of selected cells for further downstream applications.This conjugate is especially recommended for low-affinity or low-expressed targets.
Vector Laboratories Glycoprotein Eluting Solution for GlcNAc or Chitin-Binding Lectins 100 ml
This Glycoprotein Eluting Solution can be used to elute glycoproteins from agarose-bound N-acetylglucosamine- or chitin-binding lectins. The neutral pH of this solution maximizes the yield of eluted glycoproteins and preserves the activity of the agarose-bound lectins for repeated use. All components of this ready-to-use solution can be subsequently removed by dialysis. This Glycoprotein Eluting Solution offers convenience and superior recovery over standard sugar solutions.
IBA LifeSciences StrepMAB-Classic monoclonal antibody with HRP conjugate; 75 µl
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.The absorption/emission maxima of the fluorescent dyes are:• DY-488: 493/518 nm• DY-549: 550-560/575 nm• DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
Anatrace NG310-CH210 - 10:1 LMNG/CHS Pre-Made Solution
Lauryl Maltose Neopentyl Glycol (NG310) and Cholesteryl Hemisuccinate (CH210) Solution • LMNG/CHS Solution • Lauryl Maltose Neopentyl Glycol (50 mg/ml):CHS-TRIS (5 mg/ml) SolutionCAS Number: LMNG: [1257852-96-2] / CHS: [102601-49-0]Formula Weight: LMNG: 1005.19 / CHS: 607.9
STEMCELL Technologies "The Big Easy" EasySep™ Magnet, For isolating: 1 Unit
"The Big Easy" EasySep™ Magnet is designed for cell separation procedures using EasySep™ reagents when processing a large number of cells, or when using RoboSep™. "The Big Easy" EasySep™ Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep™ Magnetic Particles without the use of columns. "The Big Easy" EasySep™ Magnet is designed to hold a standard 14 mL (17 x 95 mm) polystyrene tube.
CUBE BIOTECH PureCube Fe-NTA MagBeads | 5ml
PureCube Fe-NTA MagBeads were developed for the enrichment of phosphorylated proteins the affinity matrix is based on the spherical magnetic agarose beads, consisting og 6% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. The magnetic beads are very homogeneous in size with a medium particle diameter of 30um, yielding a high degree of reproducibility between individual purificaiton runs.
STEMCELL Technologies EasySep™ Direct Human T Cell Isolation Kit, For processing: 100 mL whole blood processed
This cell separation kit can be used to isolate highly purified T cells directly from human whole blood by immunomagnetic negative selection.This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for functional assays, flow cytometry or DNA/RNA extraction.
STEMCELL Technologies EasySep™ Direct Human Eosinophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Eosinophil Isolation Kit isolates functional, highly purified eosinophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
STEMCELL Technologies EasySep™ Mouse T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse T Cell Isolation Kit is designed to isolate T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-T cells and streptavidin-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.This product replaces the EasySep™ Mouse T Cell Enrichment Kit (Catalog #19751) for even faster cell isolations.
IBA LifeSciences Strep-TactinXT 4Flow high capacity resin as 50% suspension; 50 ml
Strep-TactinXT 4Flow high capacity is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity, the resin is recommend for all protein classes, especially for low abundant proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow high capacity allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography.
Izon Science Usa Ltd qEVoriginal/35nm Gen 2 Column, 35 nm pore size, 5-pack, 500 μL sample loading volume, proprietary agarose resin, enhanced separation performance
qEVoriginal/35nm Gen 2 Column, 35 nm pore size, 5-pack, 500 μL sample loading volume, proprietary agarose resin, enhanced separation performance
Cellecta Inc LentiFuge Viral Concentration Reagent, 1 ml, 1000X solution, sterile, cell culture grade, non-toxic, concentrates virus from 1 L of supernatant
LentiFuge Viral Concentration Reagent, 1 ml, 1000X solution, sterile, cell culture grade, non-toxic, concentrates virus from 1 L of supernatant