Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Human Naïve CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve CD4+ T Cell Isolation Kit is designed to isolate naive CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes and dextran-coated magnetic particles. CD45RO+ cells are targeted for removal with a biotinylated anti-CD45RO antibody, and a bispecific Tetrameric Antibody Complex that recognizes biotin and dextran. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For even faster cell isolations, we recommend the new EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (17555) (https://www.stemcell.com/products/easysep-human-naive-cd4-t-cell-isolation-kit-ii.html) which isolates cells in just 11 minutes.
STEMCELL Technologies EasySep™ Human CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human CD8+ T Cell Enrichment Kit (Catalog #19053) for even faster cell isolations.
VWR International Stilla® Ruby Chip Pack of 12
The Ruby Chip features cutting-edge, patented microfluidic technology that integrates the entire digital PCR workflow into a single fully automation-compatible consumable.Capacity: 16 samples/chipInput volume: 5 µl/chamberDroplets per sample: Up to 17000Dynamic range: ∼4.7 logFully compatible with integration to automated liquid handling equipment
Active Motif Histone Extraction Kit
The Histone Extraction Kit provides a simple method to acid precipitate histones from cell culture, tissue and primary cells (e.g. T-cells) while preserving histone post-translational modifications. The highly basic nature of histone proteins often precludes them from being isolated using traditional methods for protein extraction. Instead, histones must be isolated under acidic conditions to retain solubility. The inclusion of inhibitors helps to preserve histone modifications such as acetylation, methylation and phosphorylation. Each Histone Extraction Kit contains enough reagents for extractions from 2 x 96-well plates (192 rxns), or 100 rxns from cultured cell lines and tissues. Kits include buffers, protease inhibitors, phosphatase inhibitors, deacetylase inhibitors and optimized protocols for efficient recovery of core histones (H2A, H2B, H3 & H4). For more complete information, please visit the Active Motif website.
STEMCELL Technologies EasySep™ APC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies by positive selection. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ APC Positive Selection Kit (Catalog #18453) for even faster cell isolations.
Protifi LLC S-Trap micro kit (10x ≤ 100 ug)
S-Trap sample processing is now available in a full kit format. The kit includes lysis, wash, and digestion buffers. The user must supply HPLC grade MeOH.
Norgen Biotek Corp AAV Purification Maxi Slurry Kit, 1-10 preps, Spin column chromatography, Cell pellet/culture media/mixed input, 45 mL - 90 mL input volume per column, Up to 900 mL total input volume, 2.5 - 3.5 hours preparation time, 1 mL - 10 mL minimum elution volume, Up to 90% AAV recove...
AAV Purification Maxi Slurry Kit, 1-10 preps, Spin column chromatography, Cell pellet/culture media/mixed input, 45 mL - 90 mL input volume per column, Up to 900 mL total input volume, 2.5 - 3.5 hours preparation time, 1 mL - 10 mL minimum elution volume, Up to 90% AAV recovery
BIOSEARCH TECHNOLOGIES INC (Lucigen) MPC Protein Precipitation Solution, 500 mLA simple solution and method for the removal of proteins from cell lysates prior to nucleic acid purification. To be used with the MasterPure Kits
This reagent is designed for use with the MasterPure™ kit protocols only.
Epigentek Inc EpiQuik Nuclear Extraction Kit (100 assays)
For isolation of nuclear proteins from cells or tissues while keeping enzymatic activity intact
Zymo Research Corporation His-Spin Protein Miniprep™ Kit (10 Preps)
The simplified procedure is based on ourinnovative protein purification chemistry and customdesigned fast spin columns. Up to 1 mg of His taggedprotein can be purified in 5 minutes and eluted in aslittle as 100 ul of His-Elution Buffer. The purifiedprotein can be used directly for enzymatic assays,protein biochemical analyses, SDS-PAGE and otherapplications. The product has been optimized formaximal protein purity: a single protein band is visibleby Coomassie blue staining on SDS-PAGE gel. The straightforward spin wash elute protocol dramatically simplifies protein purification: get results in minutes, not hours.
STEMCELL Technologies EasySep™ PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ PE Positive Selection Kit (Catalog #18557) for even faster cell isolations.
Aat Bioquest HIS Lite™ OG488-Tris NTA-Ni Complex, green fluorescent probe, 100 μg, suitable for polyhistidine-tagged proteins, fluorescent detection
HIS Lite™ OG488-Tris NTA-Ni Complex, green fluorescent probe, 100 μg, suitable for polyhistidine-tagged proteins, fluorescent detection