Protein Purification Reagents and Kits
Molecular Dimensions SG1 (SHOTGUN) SCREEN HT-96
Protein Crystallography. SG1 (SHOTGUN) SCREEN HT-96 (96 x 1.1mL): 96 conditions that occur with highest, non-redundant frequency amongst all PDB deposits. SG1 uses the ShotGun approach to gather all the most successful conditions from all the early commercially available screens.
Biotium Indo-1, AM Ester, 10x100 ug
Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading. Indo-1/AM itself does not bind Ca2 , but it is readily hydrolyzed to Indo-1 by endogenous esterases once it enters cells. Properties: Light yellow solid soluble in DMSO; Store at -20°C and protect from light, especially in solution; C47H51N3O22; MW: 1010; [112926-02-0]
Biochain Institute Inc Membrane Protein - Human Adult Normal Tissue: Colon descending, 0.1 mg/PK
Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.
Tosoh Bioscience LLC TSKGEL PHENYL-5PW GL 5X50
Column, Hydrophobic Interaction Chromatography; Tosoh TSKgel Phenyl-5PW Glass; Media: Polymer; Particle: 10 micrometer; Pore Size: 1000 angstrom; 5mm I.D. x 5cm Length; Glass
Bio Rad Laboratories TEFLON FEP TUBING
Tubing, FEP; Bio-Rad; PTFE; For use w/BioLogic DuoFlow system, other chromatography systems 0.125 in./3.2mm OD x 0.062 in./1.6mm ID; 15 ft./4.6 m; For pre-pump buffer inlet lines to pumpheads
Dojindo Molecular Technologies Inc BICINE (100G), Good's buffer, CAS: 150-25-4, pka=8.35, Active pH 7.7-9.1, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Tosoh Bioscience LLC TSKGEL ODS-120T 4.6X150
Column, Reversed Phase Chromatography; Tosoh TSKgel ODS-120T; Media: Silica; Particle: 5 micrometer; Pore Size: 120 angstrom; 4.6mm I.D. x 15cm Length; Stainless Steel
Tosoh Bioscience LLC TSKGEL DEAE-NPR 4.6X35
Column, Nonporous (NPR) HPLC; Anion Exchange; Tosoh TSKgel DEAE-NPR; Media: Nonporous polymer; Particle: 2.5 micrometer; 4.6mm I.D. x 3.5cm Length
Molecular Dimensions CLEAR STRATEGY SCREEN 1 HT-96
Protein Crystallography. Clear Strategy Screen I HT-96 (96 x 1.1mL): allows users to easily interpret results & plan optimization. In the HT-96 format, the Clear Strategy I reagents are premixed at 4 pH values (pH's 5.5, 6.5, 7.5 and 8.5).
Molecular Dimensions CYSTALIZATION SCREEN 96 X 10ML
Protein Crystallography. ProPlex screen (96 x 10mL) is formulated for the crystallization of Protein comPlexes. Based on results of Protein Complex Crystallization Database. Satisfies dual requirements: -Maintain protein-protein interactions, -Reduce solubility of complex.
ILEX LIFE SCIENCES LLC EndoTrap HD 5/1, 5 mL resin column, Affinity chromatography endotoxin (LPS) removal kit
EndoTrap HD endotoxin removal products, manufactured in Germany by LIONEX, are intended for in vitro quantitative removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts, phage, and nucleic acids. EndoTrap HD can be used in small scale processes for R&D and large scale manufacturing processes. It can be applied in early or late biomanufacturing process steps. EndoTrap HD is based on hydrophilic, dimensionally stable affinity matrix with excellent pressure/flow characteristics.Method: affinity chromatography.Format: pre-filled gravity flow columns, pre-filled FPLC columns, or bulk resin.LPS Binding Capacity: > 5,000,000 EU/mL.LPS Removal Rate: > 99.99%.Sample Recovery Rate: > 95%.pH Sample Range: 4-10.Non-toxic, no cytotoxic effects.Re-use more than 10 times.Suitable for high-salt conditions.The EndoTrap HD Leakage ELISA is available for quantitative detection of leached ligand from EndoTrap HD resin.