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A DNA sequence encoding the Varicella-zoster virus (VZV) / HHV-3 gE / glycoprotein E (Q9J3M8) (Met1-Tyr538) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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