Cell Biology Kits
Cayman Chemical MItoCheck CItrate Synthase A
The condensation of the dicarboxylate oxaloacetate and acetyl CoA to the tricarboxylate citrate is catalyzed by citrate synthase. It is within this reaction that carbon molecules (as acetyl CoA) obtained from pyruvate oxidation are fed into the tricarboxylic acid (TCA or citric acid) cycle. As a mitochondrial enzyme, citrate synthase is commonly used as a normalization factor for mitochondrial protein, but can also be used as a biomarker for mitochondrial content in a tissue homogenate. Cayman's MitoCheck Citrate Synthase Activity Assay Kit allows for the simple and convenient determination of citrate synthase activity from isolated mitochondria, tissue, or cell homogenates. This assay measures the production of SH-CoA by monitoring the absorbance of Citrate Synthase Developing Reagent at 412 nm in a convenient 96-well format.
Cayman Chemical MItoCheck Complex I ActIvIty
Complex I (NADH dehydrogenase/NADH:ubiquinone oxidoreductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the reduction of ubiquinone (Q) to form ubiquinol (QH2), and ultimately the reduction of the terminal electron acceptor, O2. During the passage of electrons from NADH to Q, the translocation of four protons (H+) from the mitochondrial matrix to the intermembrane space occurs, contributing to the chemiosmotic proton gradient, which is required for oxidative phosphorylation. Cayman's MitoCheck Complex I Activity Assay allows for the activity of complex I to be determined without the need to isolate mitochondria or pre-incubate with antibodies. The rate of NADH oxidation is measured by a decrease in absorbance at 340 nm and is proportional to the activity of complex I.
Cayman Chemical MItoCheck Complex IV ActIvIt
Complex IV (cytochrome c oxidase) is the terminal electron acceptor in the mitochondrial electron transport chain. Complex IV functions by oxidizing cytochrome c and completing a four-electron reduction of oxygen (O2) to form water. During this process, two H+ are translocated from the mitochondrial matrix to the intermembrane space, contributing the mitochondrial membrane potential required for ATP synthesis. Common inhibitors of complex IV include potassium cyanide, azide, O2 limitation, nitric oxide, and carbon monoxide. Cayman’s MitoCheck Complex IV Activity Assay is designed to measure the direct oxidation of cytochrome c by complex IV, in an isolated bovine heart mitochondrial system that is supplied within the kit.
Electron Microscopy Sciences Aurion Carboxyl Functionalized Gold Nanoparticles, 10 nm 5 ML
This product has been developed to facilitate conjugating gold nanoparticles to molecules that cannot be conjugated via the classic direct adsorption method. The product is especially suited for covalent conjugation of small ligands. Aurion Gold Nanoparticles (Carboxyl Functionalized) are polyethylene glycol-carboxyl stabilized gold nanoparticle solutions based on defined particle sizes of 6, 10, 15 and 25nm.
Particle size: 10 nm
Cayman Chemical MItoCheck Complex V ActIvIty
An allosteric inhibitor of soluble adenylyl cyclase (IC50 50 11 UM) inhibits the activity of mitochondrial complex IV in wild-type but not sAC knockout MEFs at 50 UM reduces ischemia and reperfusion-induced swelling of and ATP content decreases in rat liver mitochondria at 1.2 mg/kg
Cayman Chemical MItoCheck Complex II ActIvIt
Complex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to fumarate, and in the process reduces ubiquinone (Q) to ubiquinol (QH2). Ultimately, oxidation of succinate will lead to reduction of O2, the terminal step in mitochondrial respiration. Cayman’s MitoCheck Complex II Activity Assay allows for the activity of complex II to be determined in isolated mitochondria. As complex II oxidizes succinate, electrons are passed to an analog of ubiquinone and then on to DCPIP, which, when oxidized, absorbs in the 600 nm range. The absorbance of DCPIP will decrease upon reduction. Thus, Complex II activity is measured as a decrease in absorbance at 600 nm over time.
Hach Company Tubing With Anti-Diffusion Tip for AT Titrator - Delivery Tubing Accessory
Replacement delivery tubing with an anti-diffusion tip designed for use with AT series titrators. The anti-diffusion tip reduces reagent diffusion during dispensing to help maintain titration accuracy and repeatability.
- Prevents diffusion during reagent delivery.
- Designed for use with AT titrator series.
- Serves as a replacement delivery tubing accessory for titration systems.
- Helps maintain titration accuracy and repeatability.
SURFACE MEASUREMENT SYSTEMS NA PAYNE DIFFUSION CELL 18MM ALUM
NC3779137 PAYNE DIFFUSION CELL 18MM ALUM
MilliporeSigma NUCLEAR EXTRACTION KIT
Nuclear Extraction Kit, Contains Cytoplasmic Lysis buffer, Nuclear extraction buffer, PBS packets, DTT, 1M, Protease inhibitor cocktail, Detergent, 10%, Nuclear extraction kit is shipped and stored at -20 deg.C, 100 extractions
American Educational Products Osmosis Apparatus Kit
Observe a dramatic demonstration of the principle of osmosis.