Substrates
Cayman Chemical EnTRopeptIdase FluorogenIc
A substrate for enteropeptidase; enteropeptidase recognizes amino acid sequence DDDDK and cleaves after the lysine residue, releasing AFC, which displays ex/em spectra of 400/505 nm
CPC Scientific DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS (trifluoroacetate salt) 5MG
Useful FRET substrate for a continuous fluorescence-based assay of the malaria aspartyl protease (plasmepsin). The peptide sequence ERNleFLSFP is derived from the cleavage site present in hemoglobin, with Nle as a substitution for Met to avoid potential oxidation related problems.SEQUENCE: DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS (trifluoroacetate salt)ONE-LETTER SEQUENCE: ERNleFLSFPMOLECULAR FORMULA: C76H98N16O15S1MOLECULAR WEIGHT: 1507.78STORAGE CONDITIONS: -20 5 CCAS REGISTRY NUMBER: [263718-22-5]SYNONYMS: RESEARCH AREA: AntimicrobialREFERENCES:M.Pennington et al., Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries, 5th International Symposium, London, p. 367, R.Epton, ed., Mayflower Scientific, (1998)S.Jiang et al., Antimicrob. Agents Chemother., 45, 2577 (2001)M.Stadler et al., J. Antibiot., 58, 775 (2005)SKU(s): MALA-001A, MALA-001B
Bachem FA-Ala-Arg-OH
FA-Ala-Arg-OH, 76079-06-6, C16H23N5O5, Mr 365.39, 50mg. Substrate for human plasma carboxypeptidase N and membrane-bound carboxypeptidase D.
Medchemexpress LLC Gly-Arg-AMC | 65147-19-5 | 99.4% | 388.42 | 5 MG
Gly-Arg-AMC is a high-purity peptide substrate specifically designed for studying Dipeptidyl Aminopeptidase 1 (DPAP1) activity. It is provided in a solid, white to off-white appearance and is soluble in both DMSO and water. This substrate is essential for biochemical assays and research involving DPAP1, with specific storage recommendations to ensure long-term stability.
- Functions as a specific peptide substrate for DPAP1
- Supplied in a solid, white to off-white form
- High purity
- Soluble in DMSO and water
- Requires sealed storage away from moisture and light for optimal stability
- Stable as a powder at -80°C for 2 years or -20°C for 1 year
- Stable in solvent at -80°C for 6 months or -20°C for 1 month
Cayman Chemical PKC Pseudosubstrate InhIbIto
A synthetic peptide that corresponds to amino acid residues of the pseudosubstrate domain of PKCθ
Cayman Chemical Pntothenate-AMC 10mg
A fluorogenic substrate for vanin-1; AMC is released upon enzymatic cleavage by vanin-1 and its fluorescence can be used to quantify vanin-1 activity; ex/em = 340-360/440-460 nm, respectively
Medchemexpress LLC Z-Gly-Pro-Arg-AMC hydrochloride | 201928-42-9 | 99.2% | 656.13 | 1 MG
Z-Gly-Pro-Arg-AMC hydrochloride is a fluorescent substrate for trypsin and cathepsin K. This compound can be utilized to determine the activity of both trypsin and cathepsin K enzymes.
- Fluorescent trypsin and cathepsin K substrate
- Used to determine trypsin and cathepsin K activity
Medchemexpress LLC N-acetyl-L-tryptophyl-L-leucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-alaninamide | 1104011-59-7 | 99.8% | 587.67 | C32H37N5O6 | 5MG
Ac-WLA-AMC is a fluorogenic peptide substrate selective for the chymotrypsin-like (β5) subunit of the 20S constitutive proteasome. Upon enzymatic cleavage the 7-amino-4-methylcoumarin (AMC) fluorophore is released, enabling sensitive fluorometric measurement of proteasome β5 activity in biochemical assays. Supplied as a high-purity solid for research use.
Medchemexpress LLC Z-DEVD-AFC | 1135416-11-3 | 98.1% | 821.71 | 10 MG
Z-DEVD-AFC is a cell-permeant substrate for caspase-3, which causes a shift in fluorescence upon cleavage of the AFC fluorophore.
- Can be used to detect caspase-3-like enzyme activity.
- For research use only.
Medchemexpress LLC MBP-MAPK-Substrate | 138028-00-9 | 99.6% | 967.09 | 5 MG
MBP MAPK Substrate is utilized as an exogenous substrate for MAPK. It is intended for research use only, and not for sale to patients.
- Used as an exogenous substrate for MAPK
- Appearance: solid
- Color: white to off-white
- Sequence: Ala-Pro-Arg-Thr-Pro-Gly-Gly-Arg-R
- Sequence shortening: APRTPGGRR
- Target: p38 MAPK
- Pathway: MAPK/ERK pathway
Medchemexpress LLC Z-DEVD-AFC | 1135416-11-3 | 98.1% | 821.71 | 5 MG
Z-DEVD-AFC is a cell-permeant substrate for caspase-3. It causes a shift in fluorescence upon cleavage of the AFC fluorophore, allowing for detection of caspase-3-like enzyme activity. This product is for research use only.
- Cell-permeant substrate for caspase-3
- Fluorescence shift upon AFC fluorophore cleavage
- Detects caspase-3-like enzyme activity
- White to off-white solid
- Emission (Em): 500
- Excitation (Ex): 380
Medchemexpress LLC Z-leu-leu-glu-AMC | 348086-66-8 | MFCD01074989 | 99.9% | 664.75 | C35H44N4O9 | 10MM 1ML
Z-Leu-Leu-Glu-AMC is a fluorogenic tripeptide substrate used to measure postacidic-like (peptidylglutamyl-peptide-hydrolyzing) activity of the 20S proteasome. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for proteasome activity assays, kinetic studies, and inhibitor screening. The product is available as a ready-to-use solution in DMSO and in multiple solid pack sizes for custom preparations.
- Fluorogenic substrate that releases AMC upon cleavage.
- Sensitive assay readout suitable for kinetic measurements.
- Provided as ready-to-use 10 mM solution in DMSO or as solids for custom preparation.
- High purity confirmed by LCMS.
- Compatible with 20S and 26S proteasome activity assays.
Cayman Chemical ALP Substrate 50mg
ALP Substrate has been tested and formulated to work exclusively with Cayman’s Alkaline Phosphatase Colorimetric Activity Assay Kit (Item No. 701710).
Medchemexpress LLC D-luciferin | 2591-17-5 | MFCD00042929 | >99.0% | 280.32 g/mol | C11H8N2O3S2 | 5MG
D-Luciferin is the natural substrate for luciferase enzymes used in bioluminescent assays and imaging. It produces visible light upon enzymatic oxidation in the presence of ATP, enabling sensitive detection in cell-based and in vivo studies.
- High purity (≥99.0%) suitable for analytical and biological applications.
- Effective luciferase substrate for bioluminescent assays and imaging.
- Soluble in DMSO (125 mg/mL) and sparingly soluble in water unless pH is adjusted.
- Stable when stored protected from light at 4°C; solutions stable at -80°C for extended storage.
- Molecular formula C11H8N2O3S2 and molecular weight 280.32 g/mol.
Sigma Aldrich Fine Chemicals Biosciences Hippuryl-His-Leu acetate salt | 103404-54-2 | 500MG
Hippuryl-His-Leu acetate salt | Purity: >=98% (HPLC) | Mol Wt: 429.47 | 103404-54-2 | 500MG