Substrates
Ubpbio A FRET-based fluorogenic substrate of Papain-like protease (PLPro) from SARS viruses, including SARS-CoV-2. PLPro also has deubiquitinase activity. 5MG
Dabcyl–FRLKGGAPIKGV–Edans is an internally quenched fluorogenic substrate for the SARS-CoV or MERS-CoV family Papain-like protease (PLPro or PLP) that recognizes a LXGG consensus cleavage sites. Upon PLP-mediated cleavage between glycine and alanine, the resulting APIKGV-Edans fluorescence can be monitored at the excitation/emission wavelengths of 355/538 nm, respectively. In addition, PLP has deubiquitinating and deISG15ylation activities.
Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl beta-D-glucopyranoside >=98% (TLC) | 2492-87-7 | MFCD00006593 | 25G
4-Nitrophenyl beta-D-glucopyranoside >=98% (TLC) | Purity: >=98% (TLC) | Mol Wt: 301.25 | 2492-87-7 | MFCD00006593 | 25G
Carbosynth LLC. D-Luciferin Firefly, sodium salt monohydrate, 103404-75-7, MFCD00044938, 1 g
Formula: C11H9N2NaO4S2, Molecular weight: 320.32 g/mol, 98%, Store at -15°C, Synonyms: (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt.
Sigma Aldrich Fine Chemicals Biosciences SIGMAFAST(TM) BCIP(R)/NBT tablet | 5TAB
SIGMAFAST(TM) BCIP(R)/NBT tablet | 5TAB
Prolume Ltd Coelenterazine native | 55779-48-1 | MFCD00467176 | 10 mg
Coelenterazine native | Mol Wt: 423.46 | 55779-48-1 | MFCD00467176 | 10 mg
Enzo Life Sciences Z-Arg-Leu-Arg-Gly-Gly-AMC (5 mg)
A peptide-AMC substrate for isopeptidase T based on the C-terminal residues of ubiquitin. Purity: ≥98%. Long Term Storage: -20°C.
Apexbio Technology LLC Luminescent ATP Detection Assay Kit(Synonyms: ATP Luminescence Assay Kit, ATP Bioluminescent Assay Kit, ATP Determination Kit), 200T.
Luminescent ATP Detection Assay Kit employs firefly luciferase-mediated bioluminescence for quantitative analysis of ATP concentration Luciferase catalyzes oxidation of the substrate D-luciferin in the presence of ATP resulting in emission of luminescence with intensity directly proportional to ATP levels This assay enables sensitive measurement within a linear detection range between 1 nM and 10 M ATP applicable for solution cell and tissue samples without complex extraction The detection procedure maintains stable luminescent signals for up to 30 min and samples remain compatible for further downstream analyses such as protein quantification SDS-PAGE and Western blotting
Sigma Aldrich Fine Chemicals Biosciences Adenosine 5'-phosphosulfate sodium salt >=85% | 102029-95-8 | MFCD00057011 | 5MG
Adenosine 5'-phosphosulfate sodium salt >=85% | Purity: >=85% | Mol Wt: 427.28 | 102029-95-8 | MFCD00057011 | 5MG
Seracare Life Sciences Inc DAB-C SUBSTRATE CONCENTRATE
Available as a substrate component of the HistoMark Orange immunohistochemistry substrate kit or as a stand-alone product. Requires Enhance Orange Buffer and Peroxide Solution for use.
Abcam Magenta-Gal, Chromogenic beta-galactosidase substrate, 100MG
MW 408.63 g/mol. Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product. Used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Hippuryl-Arg carboxypeptidase substrate | 744-46-7 | MFCD00055706 | 1G
Hippuryl-Arg carboxypeptidase substrate | Purity: >=98% (TLC) | Mol Wt: 335.36 | 744-46-7 | MFCD00055706 | 1G
Enzo Life Sciences NEDD8-AMC (25 µg)
NEDD8-AMC is a fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a particularly useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential. NEDD8-AMC is a fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a particularly useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential. Concentration: 1 mg/ml. Purity: ≥95% (HPLC). Formulation: Solution in DMSO. Source: Produced in E. coli. Long Term Storage: -80°C.