Substrates
Abcam 2-Chloro-4-nitrophenyl-Beta-D-lactoside, 25MG
MW 497.8 Da, Purity >98%. Highly sensitive lactosidase, yellow coloured probe that releases the product 2-chloro-4-nitrophenol (CNP). Can be monitored spectrophotometrically at 405 nm. The rate of formation of 2-chloro-4-nitrophenol is directly proportional to the lactosidase activity in the sample. The pKa of CNP is approximately 5.5.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Gly-Gly-AMC 25mg
A fluorogenic peptide substrate; used to assess the activity of bacterial proteases from P. aeruginosa and S. aureus; enzyme activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm
Enzo Life Sciences FLUOR DE LYS deacetylase substrate (50 µl)
Highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. MW: 387.4. Formulation: Supplied as a 50 mM solution (50 µl) in dimethylsulfoxide (DMSO). Purity: ≥95% (HPLC). Long Term Storage: -80°C
Bioworld TMB Stop Reagent, 450 nm, 100 mL
TMB Stop ReagentStop reagent is 0.16M sulfuric acid used to stop the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB) reaction. Horseradish peroxidase (HRP) enzyme conjugates react withTMB and peroxide to produce a blue byproduct having maximum absorbance at 605nm. Adding Stop reagent changes it to yellow color with absorption maximum at 450 nm and stabilizes the color development.Quantity: 100ml
Vector Laboratories DAB Enhancing Solution SK-4103-400
DAB Enhancing Solution, 30 ml, intensifies the DAB reaction product in stained sections. A ten-second exposure to this solution darkens the reaction product.
Biotium Coelenterazine (ALSO KNOWN AS NATIVE COE
Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator whereas Renilla luciferase is commonly used as a reporter of transcription regulation. Coelenterazine binds apoaequorin to form an aequorin complex which emits light upon calcium binding, and can measure a broad range of calcium concentrations from ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter protein and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines can also be used for chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.Coelenterazine (native): Yellow solid soluble in MeOH or EtOH; Store at -20°C and protect from light; C26H21N3O3; MW: 423; [55779-48-1]. See also other Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines (native and analog h) for in vivo use.
![Research Products International Corp NBT [Nitro Blue Tetrazolium] [2,2'-Di-P-Nitrophenyl-5, 5'-Diphenyl-3, 3'-(3,3'-Dimethoxy-4, 4'-Diphenylene)-Ditetrazolium Chloride], 100 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-N66000-0.1.JPG-250.jpg)
Research Products International Corp NBT [Nitro Blue Tetrazolium] [2,2'-Di-P-Nitrophenyl-5, 5'-Diphenyl-3, 3'-(3,3'-Dimethoxy-4, 4'-Diphenylene)-Ditetrazolium Chloride], 100 MG
Colorimetric substrate for dehydrogenases and other oxidases. NBT is used with X-Phos for colorimetric detection of alkaline phosphatase activity in blotting, immunohistochemical and cytochemistry techniques. Protect from light.
Sigma Aldrich Fine Chemicals Biosciences Fluorescein diacetate used as cell viability stain | 596-09-8 | MFCD00005062 | 5G
Fluorescein diacetate used as cell viability stain | Mol Wt: 416.38 | 596-09-8 | MFCD00005062 | 5G
Research Products International Corp 4-Nitrophenyl-B-D-Glucopyranoside, 5 Grams
Spectrophotometric substrate for detection of β-D-Glucosidase.
Free PNP: <200ppm
AG Scientific Inc IPTG, 10 G
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
AG Scientific Inc IPTG, 5 G
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
AG Scientific Inc IPTG, 25 G
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
AdipoGen L-Leucine-7-amido-4-methylcoumarin hydrochloride
L-Leucine-7-amido-4-methylcoumarin hydrochloride, AdipoGen Life Sciences. Chemical. CAS: 62480-44-8. Formula: C16H20N2O3 . HCl. MW: 324.8. Synthetic. Fluorogenic substrate for leucine aminopeptidase. Sensitivity of fluorescence detection is greatly increased over other methods such as 4-Methoxy-naphthylamide (pNA) assays. 62480-44-8
Biotium NucView 530 Caspase-3 Substrate, 1 mM in DMSO
NucView 530 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting caspase activity in intact cells in real time by either fluorescence microscopy or flow cytometry; the substrate stains apoptotic cell nuclei with orange fluorescence (Ex/Em 528/563 nm).