Substrates
Eurofins Discoverx Coelenterazine h, 1 mg. (90-0084)
This product is an optimized form of coelenterazine substrate that is significantly more sensitive to calcium, intended for use in aequorin-based luminescent calcium assays. This product is shipped as a dry powder. This product contains 1 mg of dry powder. For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx Coelenterazine h, 10 mg. (90-0084L)
This product is an optimized form of coelenterazine substrate that is significantly more sensitive to calcium, intended for use in aequorin-based luminescent calcium assays. This product is shipped as a dry powder. This product contains 10 mg of dry powder. For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Bioworld IPTG (Isopropyl-B-D-ThioGalactopyranoside) Solution, 100mM, 1 mL
Isopropyl-β-D-Thiogalactopyranoside, an inducer of β-galactosidase is used to promote the expression of proteins in cells controlled by the lac operator system. Supplied as 1ml or 10x1ml of 100mM ready-to-use solution to be used with X-gal for blue white colony screening. Techncial Information:Biotechnology Grade.Ready-to-use solution Sterile filtered (0.22μM)Dioxane freeApplication: Petriplates: Use 40 μl per Petriplates (9 cm diameter). LB agar: Use 1μl/mL of liquid LB agar medium just before pouring (~50°C).Liquid culture: Use 1μl/mL of liquid culture medium.Sold as 1mL or 10 x 1mL.
Selleck Chemical LLC D-Luciferin
D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications In an immunocompetent mouse model of ovarian cancer the use of D-luciferin substrate and firefly luciferase preserves tumour-host immune interactions since bioluminescent imaging is a more sensitive indication of tumour growth than weight gain
Medchemexpress LLC Z-Gly-Pro-AMC | 68542-93-8 | 99.8% | 463.48 g/mol | C25H25N3O6 | 10 MG
Z-Gly-Pro-AMC is a fluorogenic peptide substrate used to measure prolyl endopeptidase activity. Enzymatic hydrolysis releases the fluorescent 7-amino-4-methylcoumarin (AMC), enabling sensitive fluorometric assays.
- Hydrolyzed by prolyl endopeptidase to release 7-amino-4-methylcoumarin (AMC).
- Excitation 380 nm, emission 465 nm for fluorometric detection.
- High purity: 99.8%.
- Molecular weight 463.48 g/mol; molecular formula C25H25N3O6.
- Available as a 10 mg vial for laboratory assays.
- Suitable for enzymatic activity assays and fluorescence-based screening.
AdipoGen Dansylcadaverine, 10121-91-2, 250 mg
Dansylcadaverine (250 mg), AdipoGen Life Sciences. Chemical. CAS: 10121-91-2. Formula: C17H25N3O2S. MW: 335.46. Synthetic. Fluorescent probe for derivatization of aldehydes, ketones and carboxylic acids. Used to prepare small fluorescent biomolecules via amidation or reductive amination. Used to monitor autophagy. Functions by marking the autophagosomes, which allows researchers to follow the molecular components of the process throughout. Accumulates in autophagic vacuoles due to a combination of ion trapping and specific interactions with membrane lipids. Has biological applications in measuring cardiac autophagic flux, studying Parkinson's disease, peritoneal ovarian tumor dissemination and respiratory diseases. This fluorescent tag is preferred over other due to its specificity and simplicity in detecting autophagic vesicles. Spectral Data: ex 335nm; em518 nm in methanol.
Medchemexpress LLC HY-15926 5g , ONPG 2-Nitrophenyl β-D-galactopyranoside CAS:369-07-3 Purity:98%
Medchemexpress, HY-15926 5g ONPG 2-Nitrophenyl β-D-galactopyranoside CAS:369-07-3 Formula:C12H15NO8 Purity:98% ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Medchemexpress LLC Boc-Gln-Arg-Arg-AMC acetate | 2223665-74-3 | 99.25% | 775.85 | 10 MG
Boc-Gln-Arg-Arg-AMC acetate is a fluorogenic substrate utilized for determining protease activity. Upon hydrolysis, it releases the fluorescent product 7-amino-4-methylcoumarin (AMC), which emits a fluorescent signal under UV light. This product is for research use only.
- Fluorogenic substrate
- Used for determining protease activity
- Releases fluorescent product 7-amino-4-methylcoumarin (AMC) upon hydrolysis
- Emits fluorescent signal under UV light
Medchemexpress LLC D-luciferin potassium | 115144-35-9 | 100.0% | 318.41 | C11H7KN2O3S2 | 250MG
D-Luciferin potassium is the potassium salt of D-luciferin, the natural substrate for firefly luciferase. In the presence of ATP and appropriate cofactors it undergoes enzymatic oxidation to produce rapid, proportional chemiluminescent light, making it suitable for reporter gene assays, cell viability measurements, and sensitive ATP detection. Supplied as a high-purity solid and available in multiple pack sizes for research use.
- High-purity potassium salt for reliable luminescent assays.
- Produces rapid, proportional chemiluminescent signal.
- Compatible with luciferase-based reporter and ATP assays.
- Supplied as a solid, stable form for storage and handling.
- Available in a range of pack sizes to suit experimental needs.
Medchemexpress LLC Ac-ANW-AMC | 2357123-49-8 | 99.4% | 10 MG
Ac-ANW-AMC is a fluorogenic peptide substrate used to measure immunoproteasome β5i activity in vitro. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for kinetic or end-point assays.
- releases AMC fluorophore upon proteolytic cleavage for fluorescence detection.
- reported excitation/emission: 380 nm/460 nm; assay notes recommend 345 nm/445 nm for β5i measurements.
- high purity (99.4%) for reliable biochemical assays.
- molecular weight 588.61 g/mol; formula C30H32N6O7.
- available in small quantities (for example, 10 mg) for research use.
- store at -20°C and protect from light; in solution, store at -80°C for long-term stability.
Medchemexpress LLC Z-Leu-Leu-Glu-AMC | 348086-66-8 | MFCD01074989 | 99.5% | 664.75 | C35H44N4O9 | 25MG
Z-Leu-Leu-Glu-AMC is a fluorogenic tripeptide substrate used to measure postacidic-like (peptidylglutamyl) hydrolyzing activity of the 20S proteasome. Proteolytic cleavage releases the fluorescent 7-amino-4-methylcoumarin (AMC) reporter, enabling quantitative, sensitive measurement of proteasome activity in biochemical and enzymatic assays.
- Fluorogenic substrate for proteasome activity assays.
- Releases AMC reporter for sensitive fluorescence detection.
- Suitable for biochemical and enzymatic assay development.
- High purity for reproducible assay performance.
- Available in small research-scale quantities.
Ubpbio K48-linked polyubiquitin chain mix containing 2-8 human ubiquitin, enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
![Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 1 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660983.jpg-250.jpg)
Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 1 G
D-Luciferin Sodium Salt (1 g) is a key reagent for bioluminescence assays, commonly used to detect luciferase enzyme activity. This compound is widely used in biochemical and molecular biology applications, where it reacts with luciferase to emit light, enabling the quantification of enzyme activity. The high concentration in this 1 g bottle is suitable for extended or large-scale experiments, including cellular assays, gene expression analysis, and ATP quantification. The bioluminescence generated through this reaction offers an effective and sensitive method for detecting and quantifying luciferase activity in research and diagnostics.
Revvity Health Sciences Inc LANCE Ultra ULight JAK1 Peptide Substrate, 0.5nmole
LANCE Ultra ULight JAK1 Peptide Substrate, 0.5nmole