Substrates
Apexbio Technology LLC D-Luciferin(Synonyms: Firefly Luciferin, Luciferin, Beetle Luciferin, (S)-2-(6'-Hydroxybenzothiazolyl)-Δ2-thiazoline-4-carboxylic acid), 50mg, CAS: 2591-17-5.
D-Luciferin (CAS 2591-17-5) is a membrane-permeable bioluminescent substrate for firefly luciferase characterized by a Km of approximately 2 M In the presence of ATP luciferin undergoes oxidation and decarboxylation catalyzed by luciferase emitting measurable photons This bioluminescence enables quantification of intracellular ATP levels both in vitro and in vivo In biomedical research D-Luciferin is commonly applied for monitoring promoter-driven luciferase gene expression and as a non-invasive imaging probe in bioluminescence imaging (BLI) studies to assess tumor burden pharmacodynamics and cellular ATP content
Sigma Aldrich Fine Chemicals Biosciences 4-Methylumbelliferyl phosphate | 3368-04-5 | MFCD00016969 | 250mg
4-Methylumbelliferyl phosphate | MW: 256.15 | 3368-04-5 | MFCD00016969 | 250mg
Abcam Nitrotetrazolium blue chloride (NBT), Chromogenic substrate, 1G
MW 817.64 g/mol. Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cytidine 5'-diphosphate (CDP) sodium salt hydrate, CDP kinase substrate, 100MG
MW 469.18 g/mol, Purity >98%. CDP (nucleoside diphosphate) kinase substrate to produce CTP in support of DNA and RNA biosynthesis. Ribonucleotide reductase substrate to produce dCMP.
The product is subject to the following: Abcam Restricted Use Statement
Selleck Chemical LLC X-GAL
X-GAL (BCIG) is a widely used chromogenic -galactosidase substrate -galactosidase cleaves X-gal and produce an insoluble detectable blue compound X-Gal is applied in blue-white selection of recombinant bacterial colonies with the lac genotype
Sigma Aldrich Fine Chemicals Biosciences Albumin–fluorescein isothiocyanate conjugate, MFCD00282182, 100 mg
Protein bovine, Synonym: FITC-albumin. Albumin−fluorescein isothiocyanate conjugate has been used: as a control to compare the intracellular delivery efficiency of proteins with or without carrier, for the fluorescent visualization of proteins and cells, as a blocking agent in ELISA, as a chemical for brain perfusion and fixation, for in vivo capillary–epithelial permeability experiment, and for confocal microscopy of right ventricular (RV) vasculature in rats.
Enzo Life Sciences X-Gal (5g). CAS: 7240-90-6
Chromogenic substrate for the enzyme β-galactosidase. Alternative name: 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside. Purity: ≥98% (HPLC, TLC). Appearance: White powder. Solubility: Soluble in dimethyl formamide. Long Term Storage: -20°C.
Enzo Life Sciences MMP-substrate (fluorogenic) (1mg)
Fluorogenic substrate for several matrix metalloproteinases (MMPs). It is cleaved by MMP-1, MMP-3, MMP-7, MMP-8, MMP-9, MMP-11, MMP-12, MMP-13, and ADAM9/MDC9, but only slightly by MMP-2 and not at all by ADAM17/TACE. Ex/Em= 340nm/440nm, although 365/450 is also appropriate. Highly quenched, with strong fluorescence by Nma at 440nm once cleavage separates it from the aromatic Dnp moiety. Compatible with most fluorometers. Alternative name: Matrix metalloproteinase substrate, Dnp-Pro-beta-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(Nma)-NH2. Purity: ≥98% (HPLC). Solubility: Soluble in DMSO (at least 20mM). Long Term Storage: -20°C.
Enzo Life Sciences Z-Arg-Leu-Arg-Gly-Gly-AMC (5 mg)
A peptide-AMC substrate for isopeptidase T based on the C-terminal residues of ubiquitin. Purity: ≥98%. Long Term Storage: -20°C.
Bioworld BCIP, 1 g
BCIP Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue, insoluble product. This product then reacts to form a chromogen homodimer. This reaction may be used to amplify the signal by catalyzing the reduction of a tetrazolium salt (usually NBT) to form an intenselycolored, insoluble formazan chromogen. Technical Information:Ultra Pure GradePurity (enzymatic): >99.0%Purity (UV): >99.0%Inhibitors (enzymatic): <0.1%Solubility (0.1% phosphate buffer): Clear and haze-freeSolubility (0.10% DMF): Pass (Clear/Haze-free)Melting Point: 195-197CA260 (1mg/ml, DMF): 0.010Ref: Unger, et al., Am. J. Surg. Pathol., 10(1): 1-8, 1986.
Chem-Impex International, Inc. D-Luciferin potassium salt | MFCD00044928 | 1G
D-Luciferin potassium salt, MFCD00044928, 1G
AG Scientific Inc IPTG, 100 G
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.