Agglutinins
Sigma Aldrich Fine Chemicals Biosciences GenomePlexR WGA Reamplific
GenomePlex WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.
Sigma Aldrich Fine Chemicals Biosciences SEQPLEX I WGA Kit Whole Ge
The SeqPlex-i WGA kit allows amplification of small quantities of DNA or from degraded/highly fragmented DNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2.Step 1 In the Pre-amplification/Library Synthesis step (using the Library Preparation Reagents) the template DNA is replicated using primers comprised of semi-degenerate 3- ends with universal 5- ends. As polymerization proceeds displaced single strands serve as new templates for additional primer annealing and extension producing random overlapping replicons flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typically range from 200
Sigma Aldrich Fine Chemicals Biosciences LIPASE FROM WHEAT GERM,5G
Lipase from wheat germType I, lyophilized powder, 5-15 units/mg solid
Sigma Aldrich Fine Chemicals Biosciences SEQPLEX I WGA Kit Whole Ge
The SeqPlex-i WGA kit allows amplification of small quantities of DNA or from degraded/highly fragmented DNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2.Step 1 In the Pre-amplification/Library Synthesis step (using the Library Preparation Reagents) the template DNA is replicated using primers comprised of semi-degenerate 3- ends with universal 5- ends. As polymerization proceeds displaced single strands serve as new templates for additional primer annealing and extension producing random overlapping replicons flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typically range from 200