Avidin/Biotin Blocking Kit (SP-2001) blocks all endogenous biotin, biotin receptors, and avidin binding sites present in tissues. This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non-specifically to tissues or proteins. This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles. The Avidin D is applied first (mixed with normal blocking serum), followed by the biotin solution.